Evidence map›Paper›PMID 41761385›Full record

ArticlePlant biotechnology journal2026

Psyllid Cysteine Cathepsins Directly Cleave the Outer Membrane Protein BamD of Citrus Huanglongbing Pathogen.

Yu Du, Yu Bin, Jinhua Yang, Manman Hu, Liqin Jiang, Qiaoling Su, Shiyi Lin, Lanfeng Qu, You Li, Taiyun Wei

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant biotechnology journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Yu DuState Key Laboratory of Agriculture and Forestry Biosecurity, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
Yu BinState Key Laboratory of Agriculture and Forestry Biosecurity, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.ORCID https://orcid.org/0000-0002-6008-0079
Jinhua YangState Key Laboratory of Agriculture and Forestry Biosecurity, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
Manman HuState Key Laboratory of Agriculture and Forestry Biosecurity, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
Liqin JiangState Key Laboratory of Agriculture and Forestry Biosecurity, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
Qiaoling SuState Key Laboratory of Agriculture and Forestry Biosecurity, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
Shiyi LinState Key Laboratory of Agriculture and Forestry Biosecurity, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
Lanfeng QuState Key Laboratory of Agriculture and Forestry Biosecurity, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
You LiState Key Laboratory of Agriculture and Forestry Biosecurity, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
Taiyun WeiState Key Laboratory of Agriculture and Forestry Biosecurity, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.ORCID https://orcid.org/0000-0002-0732-9752

Funding

Fundamental and Interdisciplinary Disciplines Breakthrough Plan of the Ministry of Education of China JYB2025XDXM701National Natural Science Foundation of China Young Scientist Fund 32402347
6 · The paper itself

Abstract

Candidatus Liberibacter asiaticus (CLas), the causative agent of citrus Huanglongbing (HLB), is transmitted by Asian citrus psyllid, Diaphorina citri; however, the role of the insect's immune effectors in defending against CLas remains poorly understood. This study reveals that D. citri cathepsin L (DcCTSL1), a member of the cysteine protease family, serves as a key immune effector directly cleaving CLas outer membrane protein BamD, thereby defending against CLas infection in insect vectors. Upon CLas infection, DcCTSL1 expression is upregulated. This protease targets the CLas membrane by specifically interacting with BamD, which in turn enables DcCTSL1 to recognise the conserved Ile145-Arg146 motif of BamD. Following this recognition, DcCTSL1 cleaves the peptide bond between Arg146 and Asp147 of BamD, an action that is mechanistically dependent on the conserved catalytic triad (Cys185, His325, and Asn350) of DcCTSL1. Heterologous application of DcCTSL1 in citrus plants confers resistance to CLas. Furthermore, a homologous citrus cysteine protease also cleaves BamD via the same mechanism. Our study reveals an evolutionarily conserved defence mechanism mediated by cysteine proteases against CLas in both insect vectors and plant hosts, thus offering a novel foundation for developing protease-based strategies against HLB.

Indexed as

Bacterial Outer Membrane ProteinsCathepsin LCathepsinsCitrusHemipteraLiberibacterPlant DiseasesRhizobiaceaeAnimalsBacterial Outer Membrane ProteinsCathepsin LCathepsinsantibacterial defenceCandidatus Liberibacter asiaticuscathepsin Lcitrus HuanglongbingDiaphorina citriouter membrane protein

Identifiers

PMID41761385
PMCPMC13205610

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.