Evidence map›Paper›PMID 41760858›Full record

ArticleScientific reports2026

Development and characterization of an inducible Tensin1 deficient transgenic murine model.

Ksenija Bernau, Kelsey Holbert, Ivy S McDermott, Babita Rahar, Kade W Coulter, Madalyn A Osterhaus, Thomas J Harr, Carson Gehl, Angie Tebon Oler, Christopher Huffman and 10 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors.

Ksenija Bernau *Division of Allergy, Pulmonary and Critical Care Medicine, Department of Medicine, School of Medicine and Public Health, University of Wisconsin-Madison, 600 Highland Avenue, Madison, WI, 53792, USA. kbernau@medicine.wisc.edu.
Kelsey Holbert *Division of Allergy, Pulmonary and Critical Care Medicine, Department of Medicine, School of Medicine and Public Health, University of Wisconsin-Madison, 600 Highland Avenue, Madison, WI, 53792, USA.
Ivy S McDermottDivision of Allergy, Pulmonary and Critical Care Medicine, Department of Medicine, School of Medicine and Public Health, University of Wisconsin-Madison, 600 Highland Avenue, Madison, WI, 53792, USA.
Babita RaharDivision of Allergy, Pulmonary and Critical Care Medicine, Department of Medicine, School of Medicine and Public Health, University of Wisconsin-Madison, 600 Highland Avenue, Madison, WI, 53792, USA.
Kade W CoulterDivision of Allergy, Pulmonary and Critical Care Medicine, Department of Medicine, School of Medicine and Public Health, University of Wisconsin-Madison, 600 Highland Avenue, Madison, WI, 53792, USA.
Madalyn A OsterhausDivision of Allergy, Pulmonary and Critical Care Medicine, Department of Medicine, School of Medicine and Public Health, University of Wisconsin-Madison, 600 Highland Avenue, Madison, WI, 53792, USA.
Thomas J HarrDivision of Allergy, Pulmonary and Critical Care Medicine, Department of Medicine, School of Medicine and Public Health, University of Wisconsin-Madison, 600 Highland Avenue, Madison, WI, 53792, USA.
Carson GehlDivision of Allergy, Pulmonary and Critical Care Medicine, Department of Medicine, School of Medicine and Public Health, University of Wisconsin-Madison, 600 Highland Avenue, Madison, WI, 53792, USA.
Angie Tebon OlerDivision of Allergy, Pulmonary and Critical Care Medicine, Department of Medicine, School of Medicine and Public Health, University of Wisconsin-Madison, 600 Highland Avenue, Madison, WI, 53792, USA.
Christopher HuffmanComparative Pathology Laboratory, Research Animal Resources and Compliance, University of Wisconsin-Madison, 1710 University Avenue, Madison, WI, 53726, USA.
Beth GrayComparative Pathology Laboratory, Research Animal Resources and Compliance, University of Wisconsin-Madison, 1710 University Avenue, Madison, WI, 53726, USA.
Trevor S LangelDivision of Allergy, Pulmonary and Critical Care Medicine, Department of Medicine, School of Medicine and Public Health, University of Wisconsin-Madison, 600 Highland Avenue, Madison, WI, 53792, USA.
Kenneth M ChanDivision of Allergy, Pulmonary and Critical Care Medicine, Department of Medicine, School of Medicine and Public Health, University of Wisconsin-Madison, 600 Highland Avenue, Madison, WI, 53792, USA.
Amir ForatiDepartment of Medicine, School of Medicine and Public Health, University of Wisconsin-Madison, 600 Highland Avenue, Madison, WI, 53792, USA.
Sandra Splinter BonDurantBiotechnology Center, University of Wisconsin-Madison, 425 Henry Mall, Madison, WI, 53706, USA.
Mark E BerresBiotechnology Center, University of Wisconsin-Madison, 425 Henry Mall, Madison, WI, 53706, USA.
Kathy J KrentzBiotechnology Center, University of Wisconsin-Madison, 425 Henry Mall, Madison, WI, 53706, USA.
C Dustin RubinsteinBiotechnology Center, University of Wisconsin-Madison, 425 Henry Mall, Madison, WI, 53706, USA.
Andres F MejiaComparative Pathology Laboratory, Research Animal Resources and Compliance, University of Wisconsin-Madison, 1710 University Avenue, Madison, WI, 53726, USA.
Nathan SandboDivision of Allergy, Pulmonary and Critical Care Medicine, Department of Medicine, School of Medicine and Public Health, University of Wisconsin-Madison, 600 Highland Avenue, Madison, WI, 53792, USA.

Funding

UW COMPREHENSIVE CANCER CENTER SUPPORTP30CA014520 · NCI · UNIVERSITY OF WISCONSIN-MADISON · PI Christian Capitini · 1985 to 2026
$142.6M
Elucidating tensin1-associated signals that promote fibronectin matrix assembly and lung fibrosisR01HL146402 · NHLBI · UNIVERSITY OF WISCONSIN-MADISON · PI SANDBO, NATHAN K · 2019 to 2023
$1.7M
Optimization and development of extracellular matrix-targeting probes for PET-based assessment of lung fibrosis disease activityK01HL171464 · NHLBI · UNIVERSITY OF WISCONSIN-MADISON · PI Ksenija Bernau · 2024 to 2026
$391k
Automated Tissue MicroarrayerS10OD023526 · OD · UNIVERSITY OF WISCONSIN-MADISON · PI MATKOWSKYJ, KRISTINA A. · 2018 to 2018
$184k
NCI NIH HHS P30 CA014520NHLBI NIH HHS K01 HL171464NHLBI NIH HHS R01 HL146402NIH HHS S10 OD023526
6 · The paper itself

Abstract

Tensin1 (TNS1) is a key component of focal and fibrillar adhesions, mediating fibrillogenesis, as well as the transduction of mechanical cues and adhesive signaling. To enable further TNS1 characterization, we have developed a novel transgenic mouse that allows for temporally controlled and lineage specific knockout of TNS1. We found no differentially observed effects of TNS1 knockout on mouse health, breeding capacity, or vital organ histopathology. In contrast, RNA sequencing analysis identified 171 differentially expressed genes with Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis demonstrating differential expression in osteoclast differentiation and a number of pathways involved in host immune response. Furthermore, STRING pathway analysis showed differential regulation of genes involved in B-cell and myeloid-related signaling pathways, a number of which were validated by reverse transcription-quantitative polymerase chain reaction (RT qPCR). Loss of TNS1 in THP-1 monocyte/macrophage cell line resulted in impaired migration and phagocytosis. We also observed a trend toward increased detection of lymphocytes in the bronchoalveolar lavage within days following TNS1 knockout. This study provides a novel understanding of the phenotypic and genotypic changes that occur following knockout of TNS1 that may lead to subsequent understanding of its role in disease.

Indexed as

TensinsAnimalsCell DifferentiationCell MovementFemaleHumansMiceMice, KnockoutMice, TransgenicOsteoclastsPhagocytosisSignal TransductionTensins

Identifiers

PMID41760858
PMCPMC12979603

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.