Evidence map›Paper›PMID 41759553›Full record

ArticleMicrobiology spectrum2026

Enzyme-enhanced RNA isolation from biofilm-producing bacteria.

Samantha M Felton, Joseph M Ficarrotta, Glynis L Kolling, Jason A Papin, Bryan W Berger

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiology spectrum, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Cell Disruption and RNA Extraction fromInternational journal of molecular sciences · 2026
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Samantha M FeltonDepartment of Biomedical Engineering, University of Virginia, Charlottesville, Virginia, USA.ORCID 0000-0002-7825-8832
Joseph M FicarrottaDepartment of Biomedical Engineering, University of Virginia, Charlottesville, Virginia, USA.
Glynis L KollingDepartment of Biomedical Engineering, University of Virginia, Charlottesville, Virginia, USA.
Jason A PapinDepartment of Biomedical Engineering, University of Virginia, Charlottesville, Virginia, USA.ORCID 0000-0002-2769-5805
Bryan W BergerDepartment of Biomedical Engineering, University of Virginia, Charlottesville, Virginia, USA.ORCID 0000-0002-6135-8677

Funding

NIH HHS AI154242NIH HHS GM147257U.S. Department of Agriculture 423671-19201
6 · The paper itself

Abstract

RNA isolation is a critical first step for gene expression analysis; however, obtaining high-quality RNA from polysaccharide-rich or biofilm-producing microbial samples remains challenging. High polysaccharide content hinders cell lysis, decreases RNA yield, and reduces sample purity, limiting the reliability and accuracy of downstream techniques such as RNA-seq and RT-qPCR. In this study, we evaluated the use of polysaccharide lyase, Smlt1473, as a pre-processing step to improve RNA isolation from

Indexed as

BiofilmsPolysaccharide-LyasesPseudomonasRNA, BacterialBacterial ProteinsBacterial ProteinsPolysaccharide-LyasesRNA, BacterialbiofilmsenzymepolysaccharidesPseudomonas aeruginosaPseudomonas syringaeRNA isolationSmlt1473

Identifiers

PMID41759553
PMCPMC13055276

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.