ArticleStem cell reports2026
Reconstitution of the cellular niche requirements for primordial germ cell-like cell progression in humans.
Article in Stem cell reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
- Generation of human primordial germ cell-like cells from cumulus and blood cell-derived isogenic human induced pluripotent stem cells†.Biology of reproduction · 2026Article
- Molecular genetics of meiotic initiation in mammals.Biology of reproduction · 2026Article
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Authors and funding
7 authors.
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Abstract
Human primordial germ cell-like cells (hPGCLCs) can be specified from human-induced pluripotent stem cells (hiPSCs), offering a valuable model for human germ cell development. However, further maturation steps of hPGCLCs rely on mouse feeders, or co-culture with mouse gonadal somatic cells. Exposure of hPGCLCs to human cellular niche has not been attempted. Here, we co-cultured female hPGCLCs in two distinct niche compartments. In reconstituted fetal ovary (rOv) culture, human fetal germ cells proliferated and initiated meiosis, while hPGCLCs upregulated gonadal germ cell markers such as DDX4. Additionally, hPGCLCs were supported and matured into migratory hPGCLCs in 3D co-culture with amnion-like cells (AMLCs). Compared to rOv, hPGCLCs in PGCLC/AMLC aggregates were less prone to dedifferentiate. In both niches, stem cell factor (SCF) was crucial for the survival of hPGCLCs. Together, this work underscores that a shift in niche is required for the further germ cell development of hPGCLCs.
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