Evidence map›Paper›PMID 41758643›Full record

ArticleSTAR protocols2026

Protocol for evaluating seedling greening capacity during dark-to-light transition.

Yixuan Fan, Shangwei Zhong

Abstract read
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Yixuan FanState Key Laboratory of Gene Function and Modulation Research, Peking-Tsinghua Center for Life Sciences, School of Life Sciences, Peking University, Beijing 100871, China.
Shangwei ZhongState Key Laboratory of Gene Function and Modulation Research, Peking-Tsinghua Center for Life Sciences, School of Life Sciences, Peking University, Beijing 100871, China; Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agriculture Sciences in Weifang, Shandong 261325, China. Electronic address: shangwei.zhong@pku.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We present a protocol to evaluate greening capacity in etiolated Arabidopsis seedlings during the critical dark-to-light transition. We describe steps for sample preparation and sowing and then detail procedures for quantifying protochlorophyllide accumulation in darkness, the greening rate upon illumination, and reactive oxygen species levels as an indicator of photo-oxidative stress. This protocol can be used for screening and phenotypic quantification across genetic backgrounds. For complete details of this protocol, please refer to Zhong et al.

Indexed as

ArabidopsisSeedlingsDarknessLightOxidative StressProtochlorophyllideReactive Oxygen SpeciesProtochlorophyllideReactive Oxygen SpeciesDevelopmental biologyMicroscopyMolecular BiologyPlant sciencesSignal Transduction

Identifiers

PMID41758643
PMCPMC12955676

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.