Evidence map›Paper›PMID 41758640›Full record

ArticleSTAR protocols2026

Protocol for the generation of a human-derived nasal epithelial model and induction of tissue-resident memory-like T cells in a co-culture system.

Lisa A King, Iris van der Valk, Jasmijn S Schrumpf, Youvika Singh, Geert H Groeneveld, Pieter S Hiemstra, Anne M van der Does, Simon P Jochems, Wesley Huisman

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Lisa A KingLeiden University Center for Infectious Diseases, Leiden University Medical Center, Leiden 2333 ZA, the Netherlands. Electronic address: l.a.king@lumc.nl.
Iris van der ValkLeiden University Center for Infectious Diseases, Leiden University Medical Center, Leiden 2333 ZA, the Netherlands.
Jasmijn S SchrumpfPulmoScience Lab, Department of Pulmonology, Leiden University Medical Center, Leiden 2333 ZA, the Netherlands.
Youvika SinghLeiden University Center for Infectious Diseases, Leiden University Medical Center, Leiden 2333 ZA, the Netherlands.
Geert H GroeneveldLeiden University Center for Infectious Diseases, Leiden University Medical Center, Leiden 2333 ZA, the Netherlands; Department of Internal Medicine, Leiden University Medical Center, Leiden 2333 ZA, the Netherlands.
Pieter S HiemstraPulmoScience Lab, Department of Pulmonology, Leiden University Medical Center, Leiden 2333 ZA, the Netherlands.
Anne M van der DoesPulmoScience Lab, Department of Pulmonology, Leiden University Medical Center, Leiden 2333 ZA, the Netherlands.
Simon P JochemsLeiden University Center for Infectious Diseases, Leiden University Medical Center, Leiden 2333 ZA, the Netherlands. Electronic address: s.p.jochems@lumc.nl.
Wesley HuismanLeiden University Center for Infectious Diseases, Leiden University Medical Center, Leiden 2333 ZA, the Netherlands. Electronic address: w.huisman@lumc.nl.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Tissue-resident memory T cells (TRMs) are a distinct subset of T lymphocytes that permanently localize in non-lymphoid tissues and mediate rapid, localized immune responses. Here, we present a protocol to study human TRMs using an in vitro model system that induces TRM-like T cells. We describe steps for generating the 3D nasal epithelial model and inducing TRMs via co-culture with autologous immune cells. We then detail procedures to analyze TRMs using flow cytometry, single-cell RNA sequencing, and cytokine release.

Indexed as

Memory T CellsNasal MucosaT-LymphocytesCoculture TechniquesEpithelial CellsFlow CytometryHumanscell-based assayscell cultureflow cytometryimmunologymicroscopymodel organismssingle cell

Identifiers

PMID41758640
PMCPMC12955643

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.