ArticleVeterinary medicine and science2026
Short-Term Impact of Betaine Supplementation on Ruminal Microbial Relative Abundance, Nutrient Digestibility, Serum Metabolites, and Milk Composition in Heat-Stressed Dairy Cows.
Article in Veterinary medicine and science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
1 citing paper in PubMed.
- Rumen-host crosstalk: an amplifier of systemic pathology in heat-stressed ruminants.Frontiers in microbiology · 2026Review
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Authors and funding
13 authors.
Funding
Abstract
This short-term study evaluated the effects of dietary betaine supplementation over 4 weeks on feed intake, milk production, nutrient digestibility, blood metabolites, and ruminal microbial communities in heat-stressed Holstein cows. Thirty lactating cows were randomly divided into a control group and a treatment group supplemented with 80 g/day of natural betaine extract for 4 weeks during summer. Betaine supplementation significantly increased dry matter intake (21.0 vs. 18.7 kg/day; p ≤ 0.05) and milk yield (35.4 vs. 31.2 kg/day; p ≤ 0.05) compared to the control. Milk composition, including fat and protein content, also improved (p ≤ 0.05). Cows receiving betaine had lower rectal temperatures (p = 0.02) and respiration rates (p = 0.01), indicating reduced heat stress. Serum concentrations of inflammatory markers-ceruloplasmin, haptoglobin, and serum amyloid A-were significantly lower in the betaine group by Week 4 (p ≤ 0.05). Plasma and rumen amino acid profiles, particularly lysine and methionine, were better preserved in the betaine group (p ≤ 0.05). Ruminal concentrations of volatile fatty acids increased (p ≤ 0.05), and the abundance of beneficial microbial species, such as Fibrobacter succinogenes, Butyrivibrio proteoclasticus, and Megasphaera elsdenii, was enhanced (p ≤ 0.05). These findings suggest that betaine supplementation is an effective nutritional strategy to improve dairy cow performance, reduce inflammation, and stabilise ruminal microbiota during heat stress.
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Registered trials
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