ArticleMicrobiology spectrum2026
Integrative analysis of Poly(A)-seq and RNA-seq reveals transcriptional regulation of poly(A) tail length in tuberculosis.
Bahetibieke Tuohetaerbaike, Jie Wang, Ying Li, Liya Yue, Peihan Wang, Cuidan Li, Hao Wen, Wenbao Zhang, Jing Wang, Fei Chen and 1 more
Abstract read
In one paragraphArticle in Microbiology spectrum, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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11 authors.
Bahetibieke Tuohetaerbaike *State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Xinjiang Medical University, Urumqi, Xinjiang, China.ORCID 0000-0003-1544-9380 Jie Wang *National Genomics Data Center, China National Center for Bioinformation, Beijing, China.
Ying Li *State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Xinjiang Medical University, Urumqi, Xinjiang, China.
Liya Yue *National Genomics Data Center, China National Center for Bioinformation, Beijing, China.
Peihan WangNational Genomics Data Center, China National Center for Bioinformation, Beijing, China.
Cuidan LiNational Genomics Data Center, China National Center for Bioinformation, Beijing, China.
Hao WenState Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Xinjiang Medical University, Urumqi, Xinjiang, China.ORCID 0000-0001-7144-220X Wenbao ZhangState Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Xinjiang Medical University, Urumqi, Xinjiang, China.
Jing WangState Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Xinjiang Medical University, Urumqi, Xinjiang, China.ORCID 0000-0003-3318-2130 Fei ChenState Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Xinjiang Medical University, Urumqi, Xinjiang, China.ORCID 0000-0003-3895-6318 Xin HuState Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Xinjiang Medical University, Urumqi, Xinjiang, China.ORCID 0009-0002-6922-8372 Funding
Education Department of Xinjiang Uygur Autonomous Region-Xinjiang Universities Scientific Research Program XJEDU2025J061Funds for International Cooperation and Exchange of the National Natural Science Foundation of China 32061143024Key Research and Development Project of Hainan Province ZDYF2021SHFZ228National Natural Science Foundation of China 82060609, 82460019Natural Science Foundation of Hainan Province 822QN471, 821QN408State key Laboratory of pathogenesis, Prevention and treatment of High Incidence Diseases in Central Asia fundings SKL-HIDCA-2021-JH9, SKL-HIDCA-2021-JH10, SKL-HIDCA-2020-35, SKL-HIDCA-2020-36, SKL-HIDCA-2020-38, SKL-HIDCA-2019-45Youth Project of the Natural Science Foundation of the Department of Science and Technology of Xinjiang Uygur Autonomous Region 2025D01C321
6 · The paper itselfAbstract
Tuberculosis (TB) is a serious global health problem, and the molecular mechanisms underlying TB pathogenesis remain poorly understood to date. Differential expression of many genes is an important contributor to TB, and poly(A) tails have previously unrecognized regulatory roles in this process that may impact gene expression. In this study, we combined Poly(A)-seq and RNA-seq to preliminarily investigate the link between poly(A) tail length and gene expression regulation in TB. The results indicated that the majority of genes harbor a longer poly(A) tail in TB, and the transcript levels of these genes were likely to be precisely regulated by poly(A) tail length. Notably, we identified 22 immune/inflammation-related genes that exhibited significant variations in both poly(A) tail length and expression levels. Furthermore, we focused on CHI3L1, associated with a robust antibacterial response, and experimentally validated its poly(A) tail length and expression level, showing that alterations in CHI3L1 poly(A) tail length affect gene expression, which may be important for IMPORTANCE: Tuberculosis (TB), caused by
Indexed as
Mycobacterium tuberculosisPoly ATuberculosisGene Expression ProfilingGene Expression Regulation, BacterialHumansRNA, MessengerRNA-SeqPoly ARNA, MessengerPoly(A)-seqpoly(A) tail lengthpost-transcriptional regulationRNA-seqtuberculosis
Identifiers
PMID41758038
PMCPMC13055295
What OpenQuestion holds
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LicenceCC BY
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