ArticleApplied and environmental microbiology2026
A novel approach to enhance methane production in anaerobic digestion of waste activated sludge via pre-enrichment by a microbial consortium of degrading fungi.
Article in Applied and environmental microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Both fungi and bacteria contribute to the efficiency of activated sludge processes. However, fungal degradability has historically been overlooked in the anaerobic fermentation of waste-activated sludge (WAS). This study investigated the role of an enriched microbial consortium of degrading fungi (MCDF) in WAS fermentation. MCDF was initially enriched using a mixture of WAS and two mesophilic anaerobic sludges with chitin as the substrate. Fungal diversity and key cell wall polysaccharides, including chitin, mannan, and glucans, were identified in WAS. The addition of enriched MCDF to WAS significantly increased methane production by 35% and disrupted the floc structure through degradation of native fungi. The enriched MCDF also utilized fungal polysaccharides from four fungal species (
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