Evidence map›Paper›PMID 41757821›Full record

ArticleJBRA assisted reproduction2026

Exposure to oral contraceptives alters human endometrial stem cells culture media secretome.

Raquel Cellin Rochetti, Fernanda Bertuccez Cordeiro, Larissa Berioni Rodrigues da Silveira, Lívia do Vale Teixeira da Costa, Renato Fraietta, Kayla Jane Perkel, Fernando Prado Ferreira, Edson Guimaraes Lo Turco

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In one paragraph

Article in JBRA assisted reproduction, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Raquel Cellin RochettiDepartment of Surgery, Division of Urology, Human Reproduction Section, São Paulo Federal University, São Paulo, Brazil, CEP: 04039-060.
Fernanda Bertuccez CordeiroDepartment of Surgery, Division of Urology, Human Reproduction Section, São Paulo Federal University, São Paulo, Brazil, CEP: 04039-060.
Larissa Berioni Rodrigues da SilveiraDepartment of Surgery, Division of Urology, Human Reproduction Section, São Paulo Federal University, São Paulo, Brazil, CEP: 04039-060.
Lívia do Vale Teixeira da CostaDepartment of Surgery, Division of Urology, Human Reproduction Section, São Paulo Federal University, São Paulo, Brazil, CEP: 04039-060.
Renato FraiettaDepartment of Surgery, Division of Urology, Human Reproduction Section, São Paulo Federal University, São Paulo, Brazil, CEP: 04039-060.
Kayla Jane PerkelDepartment of Biomedical Sciences, University of Guelph, Guelph, Ontario, Canada, N1G 2W1.
Fernando Prado FerreiraDepartment of Surgery, Division of Urology, Human Reproduction Section, São Paulo Federal University, São Paulo, Brazil, CEP: 04039-060.
Edson Guimaraes Lo TurcoDepartment of Surgery, Division of Urology, Human Reproduction Section, São Paulo Federal University, São Paulo, Brazil, CEP: 04039-060.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo evaluate the effect of oral contraceptives on the secretome of endometrial mesenchymal stem cells (EnMSC) and their potential impact on endometrial plasticity.

methodsThe EnMSC were collected from menstrual shedding of five volunteers and cultured for three passages. Cells were characterized by flow cytometry and culture media was collected at the end of each passage for further secretome analysis. Quantitative analysis included detection of aminoacids, biogenic amines, acylcarnitines, lysophosphatidylcholines, phosphatidylcholines, sphingomyelins, and hexose. Data was analyzed by partial least square analysis. Potential biomarkers were analyzed by receiver operating characteristic (ROC) curve.

resultsFrom 186 metabolites quantified in the culture media of OC and non-OC groups, 15 metabolites were identified as of high discrimination between groups by the PLS-DA. The ROC curve showed that 4 out of 15 metabolites presented more than 80% of sensitivity. These metabolites are Alanine, Phosphatidylcholine (PC) aa C30:0, Glycine and PC aa C32:2, whose concentrations were higher in the OC group than in non-OC group. The Students' T-Test analysis confirmed that Alanine was significantly higher in the OC group (p=0.00176).

conclusionsThe use of OCs could affect endometrial plasticity and influence the reproductive success. This study provides a preliminary insight into the EnMSCs response to OCs based on specific metabolite signatures, which may contribute to the comprehension of mechanism associated with EnMSC and OCs.

Indexed as

Contraceptives, OralCulture MediaEndometriumMesenchymal Stem CellsSecretomeAdultCells, CulturedFemaleHumansContraceptives, OralCulture Mediacell culturecontraceptionendometriummetabolitessecretomestem cell

Identifiers

PMID41757821
PMCPMC13055182

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.