Evidence map›Paper›PMID 41757650›Full record

ArticleEnvironmental toxicology2026

Media-Dependent Growth, Stress Responses, and Metal Accumulation Patterns in Azolla imbricata (Roxb.) Nakai Exposed to As, Cd, Cu, Pb, and Zn: Individual and Combination Effects (Synergistic, Antagonistic, and Additive).

Sung-Chul Hong, Je-Sung Park, Seong-Ho Jang, Eun-Sang Jung, Byung-Gil Jung, Anisa Dea Novianti, Velmurugan Palanivel, Young-Chae Song, Arpita Kumari, Geetha Parameswaran and 1 more

Abstract read
In one paragraph

Article in Environmental toxicology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Sung-Chul HongDepartment of Bioenvironmental Energy, College of Natural Resource and Life Sciences, Pusan National University, Miryang-si, Gyeongsangnam-do, Republic of Korea.ORCID https://orcid.org/0009-0001-6493-3956
Je-Sung ParkDepartment of Bioenvironmental Energy, College of Natural Resource and Life Sciences, Pusan National University, Miryang-si, Gyeongsangnam-do, Republic of Korea.ORCID https://orcid.org/0009-0007-9162-6402
Seong-Ho JangDepartment of Bioenvironmental Energy, College of Natural Resource and Life Sciences, Pusan National University, Miryang-si, Gyeongsangnam-do, Republic of Korea.ORCID https://orcid.org/0000-0001-7187-3536
Eun-Sang JungDepartment of Bioenvironmental Energy, College of Natural Resource and Life Sciences, Pusan National University, Miryang-si, Gyeongsangnam-do, Republic of Korea.ORCID https://orcid.org/0000-0002-2553-6421
Byung-Gil JungDepartment of Environmental Engineering, College of Engineering, Dongeui University, Busan, Republic of Korea.ORCID https://orcid.org/0000-0003-3222-0684
Anisa Dea NoviantiDepartment of Bioenvironmental Energy, College of Natural Resource and Life Sciences, Pusan National University, Miryang-si, Gyeongsangnam-do, Republic of Korea.ORCID https://orcid.org/0009-0000-2585-7404
Velmurugan PalanivelCentre for Materials Engineering and Regenerative Medicine, Bharath Institute of Higher Education and Research, Chennai, Tamil Nadu, India.ORCID https://orcid.org/0000-0001-6506-1953
Young-Chae SongMajor in Environmental Engineering, Korea Maritime and Ocean University, Busan, Republic of Korea.ORCID https://orcid.org/0000-0002-2416-4785
Arpita KumariDepartment of Sciences, Amrita School of Physical Sciences, Amrita Vishwa Vidyapeetham, Coimbatore, Tamil Nadu, India.ORCID https://orcid.org/0009-0002-7547-6401
Geetha ParameswaranAmrita School of AI, Amrita Vishwa Vidyapeetham, Coimbatore, Tamil Nadu, India.ORCID https://orcid.org/0000-0002-5637-3856
Sivakumar SubpiramaniyamDepartment of Bioenvironmental Energy, College of Natural Resource and Life Sciences, Pusan National University, Miryang-si, Gyeongsangnam-do, Republic of Korea.ORCID https://orcid.org/0000-0003-3392-1474

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this study, we examined metal accumulation and biochemical responses of Azolla imbricata (Roxb.) Nakai exposed to water medium (WM) and nutrient medium (NM) under single and combined (co-exposure) treatments with five metals (As, Cd, Cu, Pb, and Zn) at 10% environmentally relevant concentrations. Marked differences between WM and NM highlighted the influence of nutrient availability on plant responses. An inverse relationship was observed, with higher biomass in NM (WM < NM) and greater metal accumulation in WM (WM > NM). Growth inhibition, reflected by reduced photosynthetic pigment contents, was accompanied by elevated stress indicators, including electrolyte leakage, proline, malondialdehyde, and anthocyanins, confirming metal-induced phytotoxicity. Metal interactions under co-exposure were predominantly antagonistic in WM and synergistic in NM. Metal accumulation patterns (mg/kg) varied across media and exposure types: under single exposure, values ranged from 83.8 (As) to 43 881 (Zn) in WM and from 11.7 (Cd) to 12 135 (Cu) in NM; under co-exposure, they ranged from 346 (Cd) to 12 688 (Cu) in WM and from 46 (Cd) to 2859 (Cu) in NM. Accumulation sequences did not correspond to exposure concentrations, indicating metal-specific and media-dependent uptake. Under co-exposure, concurrent accumulation of multiple metals was more frequent in WM than that in NM, suggesting nutrient availability constrained simultaneous uptake. Bioconcentration factor values exceeded common thresholds under certain treatments, indicating strong accumulation potential rather than definitive hyperaccumulation. Overall, these findings highlight the potential of A. imbricata for phytoremediation of metal-contaminated waters while emphasizing cautious application in agroecosystems due to potential soil-crop-food pathway transfer.

Indexed as

FernsMetals, HeavySoil PollutantsCadmiumChlorophyllCopperStress, PhysiologicalZincCadmiumChlorophyllCopperMetals, HeavySoil PollutantsZincAzolla imbricata (Roxb.) Nakaibioaccumulationinteractive effectsmetalsstress responses

Identifiers

PMID41757650
PMCPMC13656889

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.