Rapid and Visual Detection of Muscovy Duck Reovirus Using a Reverse Transcription Recombinase-Aided Amplification Assay in a Lyophilized Format for Point-of-Care Applications.

Xiuqin Chen, Shizhong Zhang, Xiaochun Luo, Guangju You, Shaoying Chen, Meiqing Huang, Shao Wang, Shilong Chen

Abstract read

In one paragraph

Article in Transboundary and emerging diseases, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Rapid and Visual Detection of Muscovy Duck Reovirus Using a Reverse Transcription Recombinase-Aided Amplification Assay in a Lyophilized Format for Point-of-Care Applications.Transboundary and emerging diseases · 2026
Article

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Xiuqin ChenInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Science, Fuzhou, Fujian, 350013, China, faas.cn.ORCID https://orcid.org/0000-0002-4027-110X

Shizhong ZhangInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Science, Fuzhou, Fujian, 350013, China, faas.cn.ORCID https://orcid.org/0009-0000-2845-9882

Xiaochun LuoLongwen District Animal Disease Control Center, Zhangzhou, 363000, Fujian, China.ORCID https://orcid.org/0009-0002-6185-0044

Guangju YouInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Science, Fuzhou, Fujian, 350013, China, faas.cn.ORCID https://orcid.org/0009-0006-3647-1121

Shaoying ChenInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Science, Fuzhou, Fujian, 350013, China, faas.cn.ORCID https://orcid.org/0000-0002-9781-9586

Meiqing HuangInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Science, Fuzhou, Fujian, 350013, China, faas.cn.ORCID https://orcid.org/0000-0002-1491-5143

Shao WangInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Science, Fuzhou, Fujian, 350013, China, faas.cn.ORCID https://orcid.org/0000-0002-9432-2961

Shilong ChenInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Science, Fuzhou, Fujian, 350013, China, faas.cn.ORCID https://orcid.org/0000-0002-6533-8905

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Muscovy duck reovirus (MDRV) causes substantial economic losses in the waterfowl industry, necessitating rapid and field-deployable diagnostic tools. In this study, we developed, for the first time, a lyophilized reagent-based reverse transcription recombinase-aided amplification (RT-RAA) assay targeting the σC gene of MDRV for point-of-care testing (POCT). The assay was optimized to operate at 39°C with a probe concentration of 100 nM. It achieved a detection limit of 1.03 × 10

Indexed as

DucksNucleic Acid Amplification TechniquesOrthoreovirus, AvianPoultry DiseasesReoviridae InfectionsAnimalsFreeze DryingPoint-of-Care SystemsRapid Diagnostic TestsRecombinasesSensitivity and SpecificityRecombinaseslyophilized reagentMuscovy duck reoviruspoint-of-care testingreal-time reverse transcription recombinase-aided amplificationvisual readout

Identifiers

PMID41757367

DOI10.1155/tbed/5924181

PMCPMC12933139

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LicenceCC BY

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.