Evidence map›Paper›PMID 41757163›Full record

ArticlemedRxiv : the preprint server for health sciences2026

Spatial transcriptomics reveals mechanism of autoimmunity driven by internalized autoantibodies.

Iago Pinal-Fernandez, Katherine Pak, Maria Casal-Dominguez, Sandra Muñoz-Braceras, Gustaf Wigerblad, Stefania Dell'Orso, Faiza Naz, Shamima Islam, Gustavo Gutierrez-Cruz, Travis B Kinder and 32 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in medRxiv : the preprint server for health sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

42 authors.

Iago Pinal-FernandezMuscle Disease Section, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, USA.ORCID 0000-0001-6338-9218
Katherine PakMuscle Disease Section, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, USA.
Maria Casal-DominguezMuscle Disease Section, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, USA.
Sandra Muñoz-BracerasMuscle Disease Section, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, USA.
Gustaf WigerbladGenomic Technology Section, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, USA.
Stefania Dell'OrsoGenomic Technology Section, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, USA.
Faiza NazGenomic Technology Section, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, USA.
Shamima IslamGenomic Technology Section, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, USA.
Gustavo Gutierrez-CruzGenomic Technology Section, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, USA.
Travis B KinderMuscle Disease Section, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, USA.
S Amara Ogbonnaya-WhittleseyMuscle Disease Section, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, USA.
Andreu Fernández-CodinaDivision of Rheumatology, Western University, London, ON, Canada.
Margherita GianniniExplorations Fonctionnelles Musculaires, Centre de Référence Des Maladies Autoimmunes Rares, Centre de Recherche en Biomédecine de Strasbourg, Rhumatologie, Hôpitaux Universitaires de Strasbourg, UR 3072, Université de Strasbourg, Strasbourg, France.ORCID 0000-0002-4834-5804
Benjamin EllezamDivision of Pathology, CHU Sainte-Justine, Department of Pathology and Cell Biology, Université de Montréal, Montréal, Québec, Canada.ORCID 0000-0002-8716-7924
Gilles LavernyUniversité de Strasbourg, CNRS, Inserm, IGBMC UMR 7104- UMR-S 1258, F-67400 Illkirch, France.
Valentine GilbartUniversité de Strasbourg, CNRS, Inserm, IGBMC UMR 7104- UMR-S 1258, F-67400 Illkirch, France.
Océane Landon-CardinalDivision of Rheumatology, Centre hospitalier de l'Université de Montréal (CHUM), Autoimmunity Research Laboratory, CHUM Research Center, Department of Medicine, Université de Montréal, Montréal, Québec, Canada.
Marie HudsonDivision of Rheumatology, Department of Medicine, Jewish General Hospital, Lady Davis Institute and McGill University, Montreal, QC, Canada.
Yves TroyanovDivision of Rheumatology, Hôpital du Sacré-Cœur de Montréal; Department of Medicine, Université de Montréal, Montréal, Québec, Canada.
Davide RandazzoLight Imaging Section, Office of Science and Technology, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, USA.
Aster KeneaLight Imaging Section, Office of Science and Technology, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, USA.
Ana Matas-GarciaMuscle Research Unit, Internal Medicine Service, Hospital Clinic, IDIBAPS, CIBER, UB, Barcelona, Spain.
Gloria GarrabouMuscle Research Unit, Internal Medicine Service, Hospital Clinic, IDIBAPS, CIBER, UB, Barcelona, Spain.
Iban AldecoaPathology Department and Neurological Tissue Bank of the Biobank. Hospital Clinic of Barcelona-CDB-IDIBAPS/FCRB-University of Barcelona, Barcelona, Spain.
Gabriela Ailen-CaballeroPathology Department and Neurological Tissue Bank of the Biobank. Hospital Clinic of Barcelona-CDB-IDIBAPS/FCRB-University of Barcelona, Barcelona, Spain.
Albert Gil-VilaSystemic Autoimmune Disease Section, Division of Internal Medicine Vall d'Hebron Universitari Hospital, Barcelona, Spain.
Ernesto TralleroDivision of Rheumatology, Rheumatology Research Group, Vall d'Hebron University Hospital, Barcelona, Spain.
Margherita MiloneDivision of Neuromuscular Medicine, Department of Neurology, Mayo Clinic, Rochester, MN, USA.ORCID 0000-0002-4338-9007
Teerin LiewluckDivision of Neuromuscular Medicine, Department of Neurology, Mayo Clinic, Rochester, MN, USA.
Elie NaddafDivision of Neuromuscular Medicine, Department of Neurology, Mayo Clinic, Rochester, MN, USA.ORCID 0000-0001-6212-1236
Gerard EspinosaUniversity of Barcelona, Barcelona, Spain.
Carmen Pilar Simeon-AznarSystemic Autoimmune Disease Section, Division of Internal Medicine Vall d'Hebron Universitari Hospital, Barcelona, Spain.
Alfredo Guillen-Del-CastilloSystemic Autoimmune Disease Section, Division of Internal Medicine Vall d'Hebron Universitari Hospital, Barcelona, Spain.
Corinna PreusseCharité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health (BIH), Department of Neuropathology, Berlin, Germany.
Felix KleefeldCharité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health (BIH), Department of Neurology, Berlin, Germany.ORCID 0000-0002-0643-7313
Nikolas BublitzDivision of Rheumatology and Systemic Inflammatory Diseases, III. Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
Werner StenzelCharité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health (BIH), Department of Neuropathology, Berlin, Germany.
Alain MeyerExplorations Fonctionnelles Musculaires, Centre de Référence Des Maladies Autoimmunes Rares, Centre de Recherche en Biomédecine de Strasbourg, Rhumatologie, Hôpitaux Universitaires de Strasbourg, UR 3072, Université de Strasbourg, Strasbourg, France.
Janet E PopeDivision of Rheumatology, Western University, London, ON, Canada.
Albert Selva-O'CallaghanSystemic Autoimmune Disease Section, Division of Internal Medicine Vall d'Hebron Universitari Hospital, Barcelona, Spain.
Jose C MilisendaMuscle Research Unit, Internal Medicine Service, Hospital Clinic, IDIBAPS, CIBER, UB, Barcelona, Spain.
Andrew L MammenMuscle Disease Section, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Autoantibody internalization has been implicated in autoimmune disease pathogenesis, yet its mechanisms, and generality across different diseases, cell types, and affected tissues remain poorly defined. Using bulk RNA sequencing, we identified reproducible, autoantibody-specific transcriptomic signatures consistent with autoantigen dysfunction in muscle biopsies from patients with anti-Mi2 dermatomyositis and anti-PM/Scl scleromyositis across independent cohorts. Electroporation of purified patient IgG into primary cultures of healthy cells was sufficient to induce the corresponding transcriptomic programs

Indexed as

anti-Kuanti-Mi2anti-PM/Sclanti-Scl70anti-U1RNPautoantibodiesmixed connective tissue diseaseMyositisoverlap syndromeRNA-sequencingscleromyositisspatial transcriptomicssystemic sclerosis

Identifiers

PMID41757163
PMCPMC12934838

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