ArticleFrontiers in chemistry2025
Molecular imaging uncovers growth media influence on biofilms' EPS production.
Article in Frontiers in chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Biofilm growth in a laboratory typically requires media rich with amino acids and other nutrients for bacterial metabolism. Microbial biofilms consist of clusters of planktonic cells grouping and secrete extracellular polymeric substance (EPS). The EPS is a complex mixture consisting of polysaccharides, fatty acids, and lipids as well as primary and secondary metabolites among other biomolecules. Choice of growth medium is important to culturing microbes, as it should allow the bacteria to replicate at rapid rates. Herein, we study the effect of media selection on biofilm culture. We investigated three growth media, including two common complex growth media, namely, Luria Broth (LB) and Tryptic Soy Broth (TSB), and a minimal growth medium, hydrogen oxidizing de-nitrifier (HOD). The latter was supplemented with glucose as the carbon energy source for aerobic growth, and nitrate was not incorporated into the media. HOD was developed to cultivate hydrogenotrophic groundwater bacteria isolated from the Hanford Site in Richland, WA. A
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