Evidence map›Paper›PMID 41756445›Full record

ArticleResearch square2026

Identification of Sialidase-Producing Bacteria in the Tsetse Gut and Characterisation of a Paenibacillus Sialidase: A Potential Tool for Paratransgenic Control of African Trypanosomes.

Youssouf Mouliom Mfopit, Judith Sophie Engel, Emmanuel Oluwadare Balogun, Mario Waespy, Petra Berger, Daniel Mbunkah Achukwi, Sen C H Ngomtcho, Mahamat A M Ibrahim, Mohammed Mamman, Gloria Dada Chechet and 4 more

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In one paragraph

Article in Research square, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Youssouf Mouliom MfopitInstitute of Agricultural Research for Development (IRAD), Yaounde, Cameroon.
Judith Sophie EngelMARUM-PANGAEA, University of Bremen, Bremen, Germany.
Emmanuel Oluwadare BalogunDepartment of Biochemistry, Ahmadu Bello University, Zaria, Nigeria.
Mario WaespyCentre for Biomolecular Interactions Bremen, Faculty of Biology and Chemistry, University of Bremen, Bremen, Germany.
Petra BergerCentre for Biomolecular Interactions Bremen, Faculty of Biology and Chemistry, University of Bremen, Bremen, Germany.
Daniel Mbunkah AchukwiTOZARD Research Laboratory, Bambili-Tubah, Bamenda, Cameroon.
Sen C H NgomtchoNational Public Health Laboratory, Ministry of Public Health, Yaoundé, Cameroon.
Mahamat A M IbrahimDepartment of Public Health, University of N'Djamena, N'Djamena, Chad.
Mohammed MammanAfrica Centre of Excellence for Neglected Tropical Diseases and Forensic Biotechnology (ACENTDFB), Zaria, Nigeria.
Gloria Dada ChechetDepartment of Biochemistry, Ahmadu Bello University, Zaria, Nigeria.
Mohammed Nasir ShuaibuDepartment of Biochemistry, Ahmadu Bello University, Zaria, Nigeria.
Junaidu KabirAfrica Centre of Excellence for Neglected Tropical Diseases and Forensic Biotechnology (ACENTDFB), Zaria, Nigeria.
Barbara Reinhold-HurekCentre for Biomolecular Interactions Bremen, Faculty of Biology and Chemistry, University of Bremen, Bremen, Germany.
Sörge KelmCentre for Biomolecular Interactions Bremen, Faculty of Biology and Chemistry, University of Bremen, Bremen, Germany.

Funding

Multi-target approach to rational design of novel therapeutics for human African trypanosomiasisK43TW012015 · FIC · AHMADU BELLO UNIVERSITY · PI BALOGUN, EMMANUEL OLUWADARE · 2021 to 2025
$577k
FIC NIH HHS K43 TW012015
6 · The paper itself

Abstract

The gut microbiota of Tsetse influences several aspects of the host physiology and vector competence. Trypanosome survival in the tsetse midgut depends on surface sialylation. We hypothesised that bacterial sialidases in the tsetse gut could interfere with parasite transmission by counteracting trypanosomal trans-sialidase activity. This study aimed to detect sialidase activity within the tsetse gut, isolate sialidase-producing bacterial strains, and to characterise the enzymatic properties of bacterial sialidases. Tsetse collected from five African countries (Cameroon, Chad, Ethiopia, Ghana and Nigeria) were screened for sialidase activity using a newly developed field-compatible assay. Detectable activity was found only in samples from Cameroon. Bacterial isolates from these samples, representing several genera (

Indexed as

Gut BacteriaPaenibacillusSialidaseTrypanosomiasisTsetse flyvector control

Identifiers

PMID41756445
PMCPMC12934896

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.