ArticleHorticulture research2026
A novel R2R3-MYB transcription factor PpMYB5 assisting Ppbbx24-del positively regulates anthocyanin biosynthesis in 'Red Zaosu' pear.
Article in Horticulture research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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1 citing paper in PubMed.
- A 32-bp deletion inHorticulture research · 2026Article
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8 authors.
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Abstract
Anthocyanins are vital pigments that play a crucial role in the coloration of various fruits. Our previous study identified a mutant Ppbbx24-del protein in the 'Red Zaosu' pear that positively regulates anthocyanin biosynthesis. However, this mutant protein exhibited nucleo-cytoplasmic localization due to the lack of the NLS domain. We hypothesized that a transcription factor in 'Red Zaosu' pear interacts with Ppbbx24-del, facilitating its nuclear translocation for regulatory function. In this study, a PpMYB5 was screened by Y2H assay using the Ppbbx24-del as bait, which was an R2R3-MYB transcription factor and significantly up-expressed in 'Red Zaosu' compared to 'Zaosu'. Pull-down, Y2H and BiFC assays confirmed that PpMYB5 could interact with both mutant Ppbbx24-del and common PpBBX24. Notably, co-expression experiments revealed that PpMYB5 facilitated the nuclear translocation of Ppbbx24-del. Transient expression assays in 'Zaosu' pear fruits demonstrated that PpMYB5 alone failed to induce anthocyanin accumulation, but its co-expression with Ppbbx24-del significantly enhanced the anthocyanin content of fruit peel compared to Ppbbx24-del alone. This synergistic effect was accompanied by significant upregulation of key anthocyanin biosynthetic genes, including
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