High-Throughput Hyperspectral and Multiplexed Super-Resolution Fluorescence Imaging by SP-STORM.

Elric Dion Pott, Meek Yang, James Ethan Batey, Joie Embree, Bin Dong

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Article in JACS Au, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

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5 authors.

Elric Dion PottDepartment of Chemistry and Biochemistry, University of Arkansas, Fayetteville, Arkansas 72701, United States.

Meek YangDepartment of Chemistry and Biochemistry, University of Arkansas, Fayetteville, Arkansas 72701, United States.

James Ethan BateyDepartment of Chemistry and Biochemistry, University of Arkansas, Fayetteville, Arkansas 72701, United States.

Joie EmbreeDepartment of Chemistry and Biochemistry, University of Arkansas, Fayetteville, Arkansas 72701, United States.

Bin DongDepartment of Chemistry and Biochemistry, University of Arkansas, Fayetteville, Arkansas 72701, United States.ORCID https://orcid.org/0000-0002-3196-0712

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Simultaneous determination of spatial location and spectral color of single molecules at large molecular density with high throughput was achieved by combining single-molecule photoswitching and optical in-hardware Fourier transformation of single-molecule emission spectra into the phasor space. The method, named as spectral phasor enabled stochastic optical reconstruction microscopy (SP-STORM), achieved simultaneous super-resolution imaging of five subcellular structures in parallel with minimum crosstalk for the first time. The high-throughput feature of SP-STORM enables these subcellular structures to be readily resolved in about one min, which is more than an order of magnitude faster than other multiplexing single-molecule localization microscopy techniques. The concept of SP-STORM is also compatible with and can be readily applicable to other super-resolution microscopy.

Indexed as

5-plexed 3D SMLM in parallelHigh throughputHyperspectral super-resolution imagingSingle molecule spectroscopySpectral phasor

Identifiers

PMID41755849

DOI10.1021/jacsau.5c01677

PMCPMC12933340

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