Evidence map›Paper›PMID 41755634›Full record

ArticleNucleic acids research2026

CRISPR/Cas9 screening with destabilized bicistronic fluorescent protein reporter revealed PABPN1 as a hub of regulators for alternative polyadenylation.

Zhijie Hu, Yuchi Liu, Yuting Han, Mengxia Li, Ke Deng, Xin Lu, Yingye Huang, Cheng Liang, Yonghua Wang, Yonggui Fu and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nucleic acids research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Zhijie HuState Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Pharmaceutical Functional Genes, Department of Biochemistry, Innovation Center for Evolutionary Synthetic Biology, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
Yuchi LiuState Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Pharmaceutical Functional Genes, Department of Biochemistry, Innovation Center for Evolutionary Synthetic Biology, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
Yuting HanState Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Pharmaceutical Functional Genes, Department of Biochemistry, Innovation Center for Evolutionary Synthetic Biology, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
Mengxia LiState Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Pharmaceutical Functional Genes, Department of Biochemistry, Innovation Center for Evolutionary Synthetic Biology, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
Ke DengState Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Pharmaceutical Functional Genes, Department of Biochemistry, Innovation Center for Evolutionary Synthetic Biology, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
Xin LuState Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Pharmaceutical Functional Genes, Department of Biochemistry, Innovation Center for Evolutionary Synthetic Biology, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
Yingye HuangState Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Pharmaceutical Functional Genes, Department of Biochemistry, Innovation Center for Evolutionary Synthetic Biology, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
Cheng LiangState Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Pharmaceutical Functional Genes, Department of Biochemistry, Innovation Center for Evolutionary Synthetic Biology, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
Yonghua WangSchool of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
Yonggui FuState Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Pharmaceutical Functional Genes, Department of Biochemistry, Innovation Center for Evolutionary Synthetic Biology, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
Anlong XuState Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Pharmaceutical Functional Genes, Department of Biochemistry, Innovation Center for Evolutionary Synthetic Biology, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.ORCID 0000-0002-1419-3494

Funding

Guangdong Science and Technology Department 2023B1212060028National Key Research and Development Program of China 2022YFA1103900National Natural Science Foundation of China 31930084National Natural Science Foundation of China 32470586National Natural Science Foundation of China 32500472National Natural Science Foundation of China 81430099National Natural Science Foundation of China 91942301
6 · The paper itself

Abstract

Alternative polyadenylation (APA) is intricately intertwined with diverse biological processes. Efficient approaches for screening the regulatory factors of specific APA events are essential to elucidate their regulation mechanisms. Here, we first engineered a destabilized bicistronic fluorescent protein reporter (dBFPR) to enhance the sensitivity of APA detection. Then, we developed a robust high-throughput screening platform for APA regulators by integrating CRISPR/Cas9, dBFPR, and fluorescence-activated cell sorting. With this method, we successfully screened the library of RNA binding proteins and found that PTBP1, ELAVL1, and DDX3X play significant roles in regulating APA and promoting cell proliferation through interaction with PABPN1, suggesting that PABPN1 is an important hub for APA regulation.

Indexed as

CRISPR-Cas SystemsLuminescent ProteinsPoly(A)-Binding Protein IPolyadenylationCell ProliferationDEAD-box RNA HelicasesGenes, ReporterHEK293 CellsHeterogeneous-Nuclear RibonucleoproteinsHumansRNA-Binding ProteinsDEAD-box RNA HelicasesHeterogeneous-Nuclear RibonucleoproteinsLuminescent ProteinsPABPN1 protein, humanPoly(A)-Binding Protein IRNA-Binding Proteins

Identifiers

PMID41755634
PMCPMC12956349

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.