Evidence map›Paper›PMID 41754575›Full record

ArticleViruses2026

Metavirome Detection and Analysis of Viruses Present in Diseased Pumpkin in Shandong, China.

Kaijie Shang, Shenglin Luan, Qian Zhao, Xuli Gao, Weiqin Zhao, Xi Duan, Lehao Li, Wenbao Liu, Weihua Zhang

Abstract read

In one paragraph

Article in Viruses, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Kaijie ShangShandong Key Laboratory of Bulk Open-Field Vegetable Breeding, Ministry of Agriculture and Rural Affairs Key Laboratory of Huang Huai Protected Horticulture Engineering, Institute of Vegetables, Shandong Academy of Agricultural Sciences, Jinan 250100, China.ORCID 0000-0002-0793-9235

Shenglin LuanShandong Key Laboratory of Bulk Open-Field Vegetable Breeding, Ministry of Agriculture and Rural Affairs Key Laboratory of Huang Huai Protected Horticulture Engineering, Institute of Vegetables, Shandong Academy of Agricultural Sciences, Jinan 250100, China.

Qian ZhaoShandong Key Laboratory of Bulk Open-Field Vegetable Breeding, Ministry of Agriculture and Rural Affairs Key Laboratory of Huang Huai Protected Horticulture Engineering, Institute of Vegetables, Shandong Academy of Agricultural Sciences, Jinan 250100, China.

Xuli GaoShandong Key Laboratory of Bulk Open-Field Vegetable Breeding, Ministry of Agriculture and Rural Affairs Key Laboratory of Huang Huai Protected Horticulture Engineering, Institute of Vegetables, Shandong Academy of Agricultural Sciences, Jinan 250100, China.ORCID 0000-0001-9814-8342

Weiqin ZhaoShandong Key Laboratory of Bulk Open-Field Vegetable Breeding, Ministry of Agriculture and Rural Affairs Key Laboratory of Huang Huai Protected Horticulture Engineering, Institute of Vegetables, Shandong Academy of Agricultural Sciences, Jinan 250100, China.

Xi DuanShandong Agriculture and Engineering University, Jinan 250100, China.

Lehao LiShandong Key Laboratory of Bulk Open-Field Vegetable Breeding, Ministry of Agriculture and Rural Affairs Key Laboratory of Huang Huai Protected Horticulture Engineering, Institute of Vegetables, Shandong Academy of Agricultural Sciences, Jinan 250100, China.

Wenbao LiuShandong Agriculture and Engineering University, Jinan 250100, China.

Weihua ZhangShandong Key Laboratory of Bulk Open-Field Vegetable Breeding, Ministry of Agriculture and Rural Affairs Key Laboratory of Huang Huai Protected Horticulture Engineering, Institute of Vegetables, Shandong Academy of Agricultural Sciences, Jinan 250100, China.

Funding

Central Guiding Fund for Local Science and Technology Development YDZX2022132National Key Research & Development Program of China 2024YFD2300700Shandong Postdoctoral Science Foundation SDZZ-ZR-202501119

6 · The paper itself

Abstract

Viral diseases pose a serious threat to pumpkin cultivation, which is an important cucurbitaceous vegetable crop. Recently, multi-virus mixed infections in plants have been continuously detected and reported. However, studies on mixed virus infections in pumpkins are limited. Through metavirome and polymerase chain reaction (PCR) analysis, we found that pumpkins exhibiting severe viral symptoms were co-infected with squash leaf curl China virus and tomato leaf curl New Delhi virus. Transcriptome analysis revealed that 2927 genes were upregulated, and 2273 were downregulated in virus-infected pumpkin plants, compared to the gene expression in healthy pumpkin plants. Cluster analysis showed that the expression of genes related to RNA silencing and the salicylic acid resistance pathway was higher in virus-infected pumpkin plants than in healthy pumpkin plants. Furthermore, quantitative real-time PCR confirmed that the expression pattern of genes related to RNA silencing and the salicylic acid resistance pathway aligned with the transcriptome sequencing results. Our findings provide a reference for investigating the mechanism of mixed infections by these two viruses to aid in the prevention and control of viral diseases in pumpkins.

Indexed as

BegomovirusCucurbitaGenome, ViralPlant DiseasesPlant VirusesChinaGene Expression ProfilingRNA InterferenceSalicylic AcidSalicylic Acidmetavirome sequencingpumpkinRNA silencingsalicylic acid resistance pathwaysquash leaf curl China virustomato leafcurl New Delhi virus

Identifiers

PMID41754575

DOI10.3390/v18020232

PMCPMC12944876

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LicenceCC BY

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