Development of a Multiplex Droplet Digital PCR Assay for Detection of Tick-Borne Pathogens.

Lijuan He, Lin Zhang, Like Duan, Xuexia Hou, Jingwen Li, Qin Hao

Abstract read

In one paragraph

Article in Pathogens (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Lijuan HeNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, Department of Spirochetosis Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.

Lin ZhangNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, Department of Spirochetosis Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.

Like DuanNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, Department of Spirochetosis Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.

Xuexia HouNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, Department of Spirochetosis Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.

Jingwen LiNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, Department of Spirochetosis Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.

Qin HaoNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, Department of Spirochetosis Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Tick-borne pathogens pose a significant threat to human health. In this study, a multiple droplet digital PCR (ddPCR) assay was developed to detect four tick-borne pathogens:

Indexed as

BorreliaMultiplex Polymerase Chain ReactionTick-Borne DiseasesTicksAnimalsCoxiella burnetiiDNA PrimersHumansReproducibility of ResultsRickettsiaSensitivity and SpecificityDNA PrimersBorrelia burgdorferi sensu latoBorrelia miyamotoiCoxiella burnetiimultiplex droplet digital PCRspotted fever group Rickettsia

Identifiers

PMID41754464

DOI10.3390/pathogens15020212

PMCPMC12943299

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.