Construction of Highly Active Interfaces on Screen-Printed Carbon Electrodes via Controllable Electrochemical Exfoliation for High-Performance Flexible Enzyme-Free Glucose Sensing.

Enzyme-free flexible glucose sensors hold great promise in the field of wearable health monitoring. However, their performance is limited by the balance between the catalytic interface activity and stability. This paper reports a strategy for interface gradient roughening of screen-printed carbon electrodes (SPCE) via controllable electrochemical exfoliation (EE). It systematically reveals the inherent relationships among the degree of EE treatment, electrode morphology, surface chemistry, and electrochemical performance. On this basis, the deposition of gold nanoparticles (AuNPs) with high density and uniform distribution is achieved, and a high-performance flexible enzyme-free glucose sensor is constructed. The study finds that EE treatment can significantly increase the true surface area of the electrode and introduce abundant oxygen-containing functional groups, thus effectively reducing the charge transfer resistance. Nevertheless, excessive exfoliation leads to the degradation of the conductive network, indicating the existence of a critical "performance window". The EE-SPCE optimized with 150 cycles has both a high active area and good electrical conductivity, providing an ideal deposition substrate for AuNPs, increasing their distribution density by approximately 158% and reducing the average particle size to 125 nm. The fabricated AuNPs/EE-SPCE sensor exhibits excellent performance in glucose detection: it has a high sensitivity of 550.766 μA·mM