Dynamic Landscape of Alternative Splicing During Early Embryogenesis of the Rhesus Monkey.

Anqi Li, Yu Zhang, Yuanyuan Zhai, Yongqiang Xing

Abstract read

In one paragraph

Article in International journal of molecular sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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1 · What the graph read from it

What it found

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2 · The registry

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4 · The record

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5 · Who and what money

Authors and funding

4 authors.

Anqi LiInner Mongolia Key Laboratory of Life Health and Bioinformatics, School of Life Science and Technology, Inner Mongolia University of Science and Technology, Baotou 014010, China.

Yu ZhangInner Mongolia Key Laboratory of Life Health and Bioinformatics, School of Life Science and Technology, Inner Mongolia University of Science and Technology, Baotou 014010, China.

Yuanyuan ZhaiInner Mongolia Key Laboratory of Life Health and Bioinformatics, School of Life Science and Technology, Inner Mongolia University of Science and Technology, Baotou 014010, China.

Yongqiang XingInner Mongolia Key Laboratory of Life Health and Bioinformatics, School of Life Science and Technology, Inner Mongolia University of Science and Technology, Baotou 014010, China.

Funding

Basic scientific research funding for universities directly under Inner Mongolia Autonomous Region 2023RCTD023The 2025 Inner Mongolia Key Laboratory of Life Health and Bioinformatics Project 2025KYPT0135The National Natural Science Foundation of China 62161039, 62371265The Natural Science Foundation of Inner Mongolia 2024JQ10, 2024QN03079

6 · The paper itself

Abstract

The rhesus macaque is one of the closest evolutionary relatives to humans, making the study of alternative splicing (AS) during its early embryonic development highly valuable for understanding human embryogenesis and related diseases. However, systematic studies in this context remain limited. Here, a comprehensive bioinformatic analysis of AS was performed using RNA-seq data spanning early rhesus macaque embryogenesis. We identified multiple previously unannotated zygotic genome activation (ZGA) genes, thereby refining the rhesus macaque ZGA gene repertoire. The landscape of AS and differential AS events (DASEs) across early stages was characterized, revealing dynamic and stage-specific regulation, with a marked increase in AS events from the 8-cell to morula stages. In addition, weighted gene co-expression network analysis identified 35 key splicing factors (SFs) involved in regulating early rhesus macaque embryonic development. Finally, we calculated the correlation between differentially expressed SFs and DASEs during the ZGA process, and identified potential regulatory relationships between several SFs (TRA2B, IGF2BP1, HNRNPAB, and MATR3) and specific DASEs. Collectively, this study provides the first systematic analysis of AS dynamics and regulation in early rhesus macaque embryogenesis, highlighting its critical role in development and offering a valuable reference for understanding AS in early human embryos.

Indexed as

Alternative SplicingEmbryonic DevelopmentMacaca mulattaAnimalsFemaleGene Expression Regulation, DevelopmentalGene Regulatory NetworksRNA Splicing FactorsRNA Splicing Factorsalternative splicingearly embryonic developmentrhesus macaquesplicing factorzygotic genome activation

Identifiers

PMID41751864

DOI10.3390/ijms27041728

PMCPMC12940521

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.