Evidence map›Paper›PMID 41751790›Full record

ArticleInternational journal of molecular sciences2026

Improving the Thermostability of the Qβ Bacteriophage Coat Protein Through Single-Site Mutation Based on Molecular Dynamics.

Meng Qu, Mingyu Li, Jing Sun, Yanhua Jiang, Wenjia Zhu, Yingying Guo, Na Li, Dapeng Wang, Lin Yao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Meng QuKey Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Ministry of Agriculture and Rural Affairs, Qingdao 266071, China.
Mingyu LiKey Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Ministry of Agriculture and Rural Affairs, Qingdao 266071, China.
Jing SunKey Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Ministry of Agriculture and Rural Affairs, Qingdao 266071, China.
Yanhua JiangKey Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Ministry of Agriculture and Rural Affairs, Qingdao 266071, China.
Wenjia ZhuKey Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Ministry of Agriculture and Rural Affairs, Qingdao 266071, China.
Yingying GuoKey Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Ministry of Agriculture and Rural Affairs, Qingdao 266071, China.
Na LiKey Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Ministry of Agriculture and Rural Affairs, Qingdao 266071, China.
Dapeng WangDepartment of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.ORCID 0000-0003-2015-0033
Lin YaoKey Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Ministry of Agriculture and Rural Affairs, Qingdao 266071, China.

Funding

Central Public-interest Scientific Institution Basal Research Fund, YSFRI, CAFS 2023TD76Central Public-interest Scientific Institution Basal Research Fund, YSFRI, CAFS 20603022025007Earmarked fund for CARS CARS-49Na-tional Key Research and Development Program of China 2017YFC1600703National Natural Science Foundation of China 32172292
6 · The paper itself

Abstract

Norovirus is a major cause of acute viral gastroenteritis in humans. Molecular biology-based detection methods play a pivotal role in ensuring accurate and specific diagnosis. The inclusion of Qβ phage particles as armored positive controls in these assays can further enhance their reliability and specificity. Herein, we discuss rational design strategies to improve the stability of Qβ bacteriophage capsid proteins armored with RNA using Discovery Studio 2019 protein design software. Amino acid mutation sites were deter-mined based on changes in folding free energy differences (ΔΔGmut). These single-site mutations were subsequently evaluated using molecular dynamics simulations. Wild-type and mutant recombinant expression plasmids were constructed and transformed into

Indexed as

AllolevivirusCapsid ProteinsMolecular Dynamics SimulationMutationEscherichia coliProtein StabilityTemperatureCapsid Proteinsarmored RNAmolecular dynamics simulationQβ phagerational designsingle-site mutationthermostability

Identifiers

PMID41751790
PMCPMC12940326

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.