Gene Expression and Immunohistochemical Analyses of c-Myc in Canine and Feline Soft Tissue Fibrosarcomas.

Canine and feline fibrosarcomas are malignant tumors of mesenchymal origin showing histological, molecular, and clinical structures similar to their human equivalent. In human medicine, cellular myelocytomatosis (c-Myc) has already been proposed as a vital gene that regulates aggressive cell growth and is a predictive marker and possible therapeutic target in fibrosarcomas. The goal of this study was to evaluate c-Myc expression in canine and feline fibrosarcomas by gene and protein analyses and correlate its expression to the histological grading system of the examined cancers. Nineteen archival formalin-fixed and paraffin-embedded canine and feline sarcoma samples were recruited. Sixteen of them were determined to be canine and feline fibrosarcomas. The samples consisted of eight cutaneous samples, four eyelid samples, three oral mucosa samples, and one vulva sample. Histopathological examinations were performed to estimate histological type, differentiation, cellularity, and mitotic count. On each sample, immunohistochemical assessment and mRNA expression were performed using an anti-c-Myc antibody and a