Evidence map›Paper›PMID 41750569›Full record

ArticleAntioxidants (Basel, Switzerland)2026

A Phycoerythrin-SOD Fluorescent Probe Enables Detection of Oxidative Stress for Assessing Astaxanthin in NAFLD.

Kun Li, Zhen Zhang, Ran Chen, Shilin Wu, Ning Yang, Jingyun Chen, Hongxiang Zhao, Pei Wang, Yunmei Yin, Meicong Xiao and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Antioxidants (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Kun LiCollege of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China.ORCID 0009-0008-8316-6511
Zhen ZhangCollege of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China.ORCID 0000-0001-8057-7624
Ran ChenCollege of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China.
Shilin WuZhejiang Provincial Key Laboratory of Multiomics and Molecular Enzymology, Yangtze Delta Region Institute of Tsinghua University, Jiaxing 314006, China.
Ning YangZhejiang Provincial Key Laboratory of Multiomics and Molecular Enzymology, Yangtze Delta Region Institute of Tsinghua University, Jiaxing 314006, China.
Jingyun ChenZhejiang Provincial Key Laboratory of Multiomics and Molecular Enzymology, Yangtze Delta Region Institute of Tsinghua University, Jiaxing 314006, China.
Hongxiang ZhaoCollege of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China.
Pei WangCollege of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China.
Yunmei YinCollege of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China.
Meicong XiaoCollege of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China.
Rongqing ZhangZhejiang Provincial Key Laboratory of Multiomics and Molecular Enzymology, Yangtze Delta Region Institute of Tsinghua University, Jiaxing 314006, China.

Funding

Yangtze Delta Region Institute of Tsinghua University LZZLX24B002
6 · The paper itself

Abstract

objectiveTo develop a superoxide dismutase (SOD) fluorescent detection probe based on Phycoerythrin (PE) from

methodsPhycoerythrin and SOD were covalently coupled using the heterobifunctional cross-linker N-Succinimidyl 3-(2-pyridyldithio) propionate (SPDP), and the probe concentration and incubation time were optimized. A NAFLD model was established in HepG2 cells induced by free fatty acids (FFAs). The fluorescence intensity of the probe was detected by flow cytometry, and the intervention effect of astaxanthin was evaluated by measuring triglyceride (TG)/total cholesterol (TC) contents and SOD activity.

resultsThe optimal conditions for the Phycoerythrin-SOD probe were determined. Astaxanthin at 20 μM significantly reduced FFA-induced TG (56.8%) and TC (63.6%) contents and restored SOD activity to 60% of that in the control group.

conclusionThe Phycoerythrin-SOD probe serves as an efficient tool for dynamic monitoring of SOD activity in NAFLD. Astaxanthin alleviates liver injury by multi-target regulation of lipid metabolism and antioxidant pathways.

Indexed as

astaxanthinfluorescent probenon-alcoholic fatty liver diseaseoxidative stressphycoerythrinSOD activity monitoring

Identifiers

PMID41750569
PMCPMC12938162

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