Evidence map›Paper›PMID 41750374›Full record

ArticleBiomolecules2026

A Simplified Strategy for Nanobody Production and Use Based on Functional GST-Nanobody Fusion Proteins.

Agustín A Burgos, Andrés Rivera-Dictter, Pablo Mendoza-Soto, Tammy P Pástor, José Munizaga, Guillermo Valenzuela-Nieto, Gonzalo A Mardones

Abstract read
In one paragraph

Article in Biomolecules, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Agustín A BurgosEscuela de Medicina, Universidad San Sebastián, Valdivia 5110466, Chile.ORCID 0009-0007-7090-9307
Andrés Rivera-DictterDepartment of Physiology, Universidad Austral de Chile, Valdivia 5110027, Chile.
Pablo Mendoza-SotoEscuela de Medicina, Universidad San Sebastián, Valdivia 5110466, Chile.ORCID 0009-0008-3624-3457
Tammy P PástorEscuela de Medicina, Universidad San Sebastián, Valdivia 5110466, Chile.ORCID 0000-0003-1424-9096
José MunizagaFacultad de Ciencias para el Cuidado de la Salud, Universidad San Sebastián, Valdivia 5110693, Chile.
Guillermo Valenzuela-NietoFacultad de Ciencias para el Cuidado de la Salud, Universidad San Sebastián, Valdivia 5110693, Chile.
Gonzalo A MardonesEscuela de Medicina, Universidad San Sebastián, Valdivia 5110466, Chile.ORCID 0000-0001-9390-5893

Funding

Fondo Nacional de Desarrollo Científico y Tecnológico 1251655
6 · The paper itself

Abstract

Nanobodies (VHHs or single-domain antibodies) are powerful affinity reagents, but their routine use is often limited by production constraints and by the lack of a conserved Fc region for secondary detection. We describe a simplified strategy in which functional GST-nanobody fusion proteins are expressed directly in the cytoplasm of

Indexed as

Glutathione TransferaseRecombinant Fusion ProteinsSingle-Domain AntibodiesEscherichia coliGreen Fluorescent ProteinsHumansGlutathione TransferaseGreen Fluorescent ProteinsRecombinant Fusion ProteinsSingle-Domain AntibodiesGST-pulldownnanobodyrecombinant proteinssingle-domain antibody

Identifiers

PMID41750374
PMCPMC12938648

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.