Evidence map›Paper›PMID 41750357›Full record

ArticleBiomolecules2026

Comparative Analysis of Lysis Buffers for Enhanced Proteomic and Glycoproteomic Profiling.

Tiantian Chu, Bo Meng, Xinyu Ji, Jinze Huang, Huanyue Liao, Rui Zhai, Xuping Shentu, Xiang Fang, Yang Zhao

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomolecules, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Tiantian ChuZhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine Technology, College of Life Sciences, China Jiliang University, Hangzhou 310018, China.
Bo MengTechnology Innovation Center of Mass Spectrometry for State Market Regulation, Center for Advanced Measurement Science, National Institute of Metrology, Beijing 100029, China.
Xinyu JiTechnology Innovation Center of Mass Spectrometry for State Market Regulation, Center for Advanced Measurement Science, National Institute of Metrology, Beijing 100029, China.
Jinze HuangTechnology Innovation Center of Mass Spectrometry for State Market Regulation, Center for Advanced Measurement Science, National Institute of Metrology, Beijing 100029, China.
Huanyue LiaoTechnology Innovation Center of Mass Spectrometry for State Market Regulation, Center for Advanced Measurement Science, National Institute of Metrology, Beijing 100029, China.
Rui ZhaiTechnology Innovation Center of Mass Spectrometry for State Market Regulation, Center for Advanced Measurement Science, National Institute of Metrology, Beijing 100029, China.
Xuping ShentuZhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine Technology, College of Life Sciences, China Jiliang University, Hangzhou 310018, China.ORCID 0000-0001-8844-4228
Xiang FangTechnology Innovation Center of Mass Spectrometry for State Market Regulation, Center for Advanced Measurement Science, National Institute of Metrology, Beijing 100029, China.
Yang ZhaoTechnology Innovation Center of Mass Spectrometry for State Market Regulation, Center for Advanced Measurement Science, National Institute of Metrology, Beijing 100029, China.ORCID 0000-0003-1444-5927

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Efficient and reproducible protein extraction is a critical prerequisite for high-quality proteomic and glycoproteomic analyses. In this study, four commonly used lysis buffers, sodium dodecyl sulfate (SDS), guanidine hydrochloride (GuHCl), urea (UA), and mammalian protein extraction reagent (MPER), were systematically evaluated within an integrated proteomic and

Indexed as

GlycoproteinsProteomeProteomicsBuffersGlycosylationGuanidineHEK293 CellsHeLa CellsHumansSodium Dodecyl SulfateUreaBuffersGlycoproteinsGuanidineProteomeSodium Dodecyl SulfateUrealysis buffersmass spectrometryN-glycosylationproteomicsSDS

Identifiers

PMID41750357
PMCPMC12938497

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.