ArticleCancers2026
Antigen-Specific Ganglioside Serological Profile of Pancreatic and Gastric Cancer Patients by Multiple TLC Overlay Assay and IR-MALDI Mass Spectrometry.
Article in Cancers, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
backgroundAltered glycosphingolipidome in cancerous tissues and cells reflects the circulatory glycosphingolipid (GSL) profiles, which is advantageous for establishing cancer biomarkers and/or unravelling GSL-associated mechanisms of immunity in cancer.
methodsHere, we combined a microscale extraction of GSLs with multiple overlay TLC assays and IR-MALDI-o-TOF MS and implemented it for the first time in serum analysis of CD75s-, CD15s-, and iso-CD75s-containing sialylated GSLs of ganglio- and neolacto-series.
resultsThis sensitive antigen-specific targeted GSL workflow enabled the identification of 80 sialylated GSLs containing the specific antigens in human sera and was applied for the investigation of clinical serum samples from gastric/stomach cancer patients (
conclusionsThis drop suggesting a role of these highly polar complex ganglioside species in evading humoral tumor immune response in the early tumor stages.
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