ArticleLight, science & applications2026
Interferometric Image Scanning Microscopy for label-free imaging at 120 nm lateral resolution inside live cells.
Article in Light, science & applications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
3 citing papers in PubMed.
- Heterogeneous Reactivity of Palladium Nanoparticles Revealed by Wavelength-Resolved Interferometric Scattering.Nano letters · 2026Article
- Lipids Contribute to Heterochromatin Condensation Revealed by Quantitative Raman-Brillouin Microscopy.JACS Au · 2026Article
- Interferometric image scanning microscopy enables label-free super-resolution imaging of live cells.Light, science & applications · 2026Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Light microscopy remains indispensable in life sciences for visualizing cellular structures and dynamics in live specimens. Yet, conventional fluorescence imaging can suffer from phototoxicity, limited labeling efficiency, or perturbation of biological function. Label-free techniques such as interferometric scattering microscopy (iSCAT) offer a powerful alternative by detecting nanoscale structures based on their light scattering, without the need for dyes or genetic tags. iSCAT has enabled high-sensitivity detection of single proteins and viruses on clean surfaces. More recently, its application to live cells has been extended by using confocal illumination and detection, allowing suppression of out-of-focus light, yielding subcellular structures with high contrast. This development laid the foundation for biologically relevant label-free imaging. Here, we introduce interferometric image scanning microscopy (iISM). This next-generation technique combines interferometric detection with image scanning microscopy to achieve about 120 nm lateral resolution while operating at tenfold lower incident illumination power per diffraction limited spot, significantly reducing photodamage while enhancing signal-to-noise and contrast. Using iISM, we are able to visualize intracellular organelles such as the endoplasmic reticulum, actin cytoskeleton, mitochondria, and vesicles in live cells at essentially unlimited observation times. Importantly, iISM can be readily combined with confocal fluorescence microscopy, enabling correlation of label-free dynamics and structural information with molecular specificity. Our approach opens new avenues for studying dynamic biological processes, such as host-pathogen interactions, intracellular trafficking, or cytoskeletal rearrangements, under label-free, near-native conditions. iISM thus offers a powerful new tool for high-resolution, low-impact imaging of live cells, paving the way for new biological insights.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.