ArticleAnalytical chemistry2026
Development of an Anti-Immunocomplex Antibody and Non-competitive Immunoassay for the Detection of Testosterone.
Article in Analytical chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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4 authors.
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Abstract
Accurate and sensitive quantification of testosterone using simple, high-throughput, and low-cost methods has remained a longstanding analytical challenge, despite the issue being highlighted by the endocrinology community more than a decade ago. Existing competitive immunoassays lack sensitivity and specificity, while reference methods based on mass spectrometry are complex, costly, and unsuitable for routine large-scale testing. Alternative approaches are needed to aid in the diagnosis of androgen deficiency and excess in men, women, and children. In this study, we report a novel anti-immunocomplex (anti-IC) antibody that recognizes a monoclonal anti-testosterone antibody bound to testosterone. The anti-IC antibody was generated using phage display, and the antibody pair was utilized in the development of a non-competitive time-resolved fluorescence immunoassay for the detection of testosterone. The assay demonstrated reliable detection within the physiological range of testosterone in men, women, and children and compatibility with plasma as a sample matrix. These findings emphasize the potential of novel anti-IC antibodies in the development of more sensitive immunoassays, offering accessible alternatives to existing methods for testosterone analysis.
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