Evidence map›Paper›PMID 41748166›Full record

ArticlePlant biotechnology journal2026

The Rice Cis-Natural Antisense Transcript NAT1850 of Pri-miR1850 Negatively Regulates Cold Tolerance by Repressing NPR3.

Yang Shen, Yan Wang, Lijia Yang, Dongpeng Li, Weifeng Dong, Xijuan Zhang, Weibing Yang, Hui Xie, Jin Liu, Shiyong Song and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant biotechnology journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Yang ShenCrop Stress Molecular Biology Laboratory, College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, China.
Yan WangCrop Stress Molecular Biology Laboratory, College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, China.
Lijia YangState Key Laboratory of Rice Biology and Breeding, Zhejiang Key Laboratory of Crop Germplasm Innovation and Utilization, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.
Dongpeng LiCrop Stress Molecular Biology Laboratory, College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, China.
Weifeng DongCrop Stress Molecular Biology Laboratory, College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, China.
Xijuan ZhangInstitute of Crop Cultivation and Tillage, Heilongjiang Academy of Agricultural Sciences, Harbin, China.
Weibing YangBeijing Academy of Agriculture and Forestry Sciences, Beijing, China.
Hui XieNational Japonica Rice Engineering and Technology Research Center, Tianjin, China.
Jin LiuRice Research Institute, Jiangxi Academy of Agricultural Sciences, Nanchang, China.
Shiyong SongState Key Laboratory of Rice Biology and Breeding, Zhejiang Key Laboratory of Crop Germplasm Innovation and Utilization, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.
Mingzhe SunCrop Stress Molecular Biology Laboratory, College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, China.
Xiaoli SunCrop Stress Molecular Biology Laboratory, College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, China.ORCID https://orcid.org/0000-0003-1134-3976

Funding

China Postdoctoral Science Foundation 2024MD763990Heilongjiang Provincial Postdoctoral Science Foundation LBHZ24027Key Research and Development Plan of Heilongjiang Province GZ2024054Major Science and Technology Research and Development Special Project of Jiangxi Province 20232ACF01001National Natural Science Foundation of China 32572284Open Research Fund of State Key Laboratory of Plant Environmental Resilience SKLPERKF2504Special Program for Centralised Guidance of Local Scientific and Technological Development ZY24QY02"Xinyan" Team Project of the Leading Talents Support Plan DQXY202402Zhejiang Provincial Natural Science Foundation LRG25C130001
6 · The paper itself

Abstract

Natural antisense transcripts (NATs) correspond to nearly 60% of annotated rice loci, however their functions are largely unknown. In this study, we characterise a rice cis-NAT (NAT1850) that completely overlaps with a rice-specific primary miRNA, pri-miR1850. Pri-miR1850, but not its mature miR1850 products, promotes the accumulation of NAT1850, while NAT1850 overexpression in turn reduces the accumulation of pri-miR1850 transcripts. A 21-nt siRNA (siR1850) derived from the pri-miR1850 transcripts is generated by cleavage of pri-miR1850-NAT1850 dsRNA and overlaps in sequence with miR1850.1 and miR1850.2. Both NAT1850 and siR1850 negatively regulate cold tolerance at both the young-seedling and booting stages. Interestingly, siR1850 targets and represses NPR3, which is also a target of miR1850.1. NPR3 interacts with the WRKY76 transcription factor and acts as a co-transcriptional activator of WRKY76 to trigger DREB1B under cold stress. Genetic evidence shows the NAT1850-siR1850 module functions in cold stress response via an NPR3-dependent manner. Furthermore, NAT1850 and siR1850 control nitrogen assimilation and rice yield in a miR1850.1-NPR3-independent pathway. Our findings reveal a regulatory mode for a pri-miRNA and its cis-NAT, and uncover their roles in balancing the cold-stress response and rice yields.

Indexed as

MicroRNAsOryzaPlant ProteinsRNA, AntisenseCold TemperatureGene Expression Regulation, PlantMicroRNAsPlant ProteinsRNA, Antisensecold tolerancegrain yieldmiR1850natural anti‐sense transcriptNPR3ricesiR1850

Identifiers

PMID41748166
PMCPMC13205835

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.