Article in Science advances, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registry
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
5 · Who and what money
Authors and funding
19 authors.
Takuya SatoDepartment of Regenerative Medicine, Yokohama City University Graduate School of Medicine, Yokohama 236-0004, Kanagawa, Japan.ORCID 0000-0002-6198-2234
Takashi YoshinoDepartment of Genome Biology, Graduate School of Medicine, Osaka University, Suita 565-0871, Osaka, Japan.ORCID 0000-0003-0002-2042
Mai OhtsukaDepartment of Regenerative Medicine, Yokohama City University Graduate School of Medicine, Yokohama 236-0004, Kanagawa, Japan.
Takahiro SuzukiLaboratory for Cellular Function Conversion Technology, RIKEN Center for Integrative Medical Sciences, Yokohama 230-0045, Kanagawa, Japan.ORCID 0000-0002-0972-6911
Takafumi MatsumuraDepartment of Regenerative Medicine, Yokohama City University Graduate School of Medicine, Yokohama 236-0004, Kanagawa, Japan.ORCID 0000-0002-0550-8589
Yuki MatsudairaDepartment of Regenerative Medicine, Yokohama City University Graduate School of Medicine, Yokohama 236-0004, Kanagawa, Japan.
Yu Ishikawa-YamauchiDepartment of Regenerative Medicine, Yokohama City University Graduate School of Medicine, Yokohama 236-0004, Kanagawa, Japan.ORCID 0009-0006-2438-619X
Shiori MaedaLaboratory for Cellular Function Conversion Technology, RIKEN Center for Integrative Medical Sciences, Yokohama 230-0045, Kanagawa, Japan.
Haruka YabukamiLaboratory for Cellular Function Conversion Technology, RIKEN Center for Integrative Medical Sciences, Yokohama 230-0045, Kanagawa, Japan.ORCID 0009-0006-3544-1326
Yoshiakira KanaiDepartment of Veterinary Anatomy, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan.ORCID 0000-0003-2116-7806
Miki InoueDepartment of Anatomy, Kurume University School of Medicine, 67 Asahi-machi, Kurume 830-0011, Fukuoka, Japan.ORCID 0000-0003-3581-9852
Yuichi ShimaDepartment of Anatomy, Kurume University School of Medicine, 67 Asahi-machi, Kurume 830-0011, Fukuoka, Japan.ORCID 0000-0002-0281-8602
Makoto TachibanaLaboratory of Epigenome Dynamics, Graduate School of Frontier Biosciences, Osaka University, Suita 565-0871, Osaka, Japan.ORCID 0000-0001-7977-1120
Shogo MatobaBioresource Engineering Division, BioResource Research Center, RIKEN, Tsukuba 305-0074, Ibaraki, Japan.ORCID 0000-0003-0474-232X
Kimiko InoueBioresource Engineering Division, BioResource Research Center, RIKEN, Tsukuba 305-0074, Ibaraki, Japan.ORCID 0000-0001-7344-4786
Narumi OgonukiBioresource Engineering Division, BioResource Research Center, RIKEN, Tsukuba 305-0074, Ibaraki, Japan.
Atsuo OguraBioresource Engineering Division, BioResource Research Center, RIKEN, Tsukuba 305-0074, Ibaraki, Japan.ORCID 0000-0003-0447-1988
Katsuhiko HayashiDepartment of Genome Biology, Graduate School of Medicine, Osaka University, Suita 565-0871, Osaka, Japan.ORCID 0000-0002-2479-2941
Takehiko OgawaDepartment of Regenerative Medicine, Yokohama City University Graduate School of Medicine, Yokohama 236-0004, Kanagawa, Japan.ORCID 0000-0002-1422-0317
Funding
No grant is acknowledged in the PubMed record.
6 · The paper itself
Abstract
Cellular interactions between germ cells and gonadal somatic cells are essential for the progression of gametogenesis. Here, we report a culture method for generating fetal testicular somatic cell-like cells (fTeSLCs) from embryonic stem cells. These fTeSLCs exhibit a transcriptomic profile closely resembling that of their in vivo counterparts, including distinct cell populations corresponding to Sertoli cells and interstitial cells. For functional assessment, interstitial cell-like cells (ICLCs) and Sertoli-like cells (SerLCs) were isolated from fTeSLCs. ICLCs differentiated into Leydig cells when cocultured with testes lacking endogenous Leydig cells, thereby restoring androgenic support. SerLCs reconstituted the seminiferous epithelium following selective ablation of endogenous Sertoli cells. Both cell types supported spermatogenesis and generated spermatids reaching the elongating stage. Notably, round spermatids derived from these reconstructed systems produced viable offspring by round spermatid injection. These findings demonstrate that fTeSLCs can generate functional testicular somatic cells, providing a valuable platform for studying testis development and spermatogenesis.
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.
Generation of functionally competent testicular somatic cells from pluripotent stem cells. · full record | OpenQuestion