Evidence map›Paper›PMID 41746729›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2026

HOP2-MND1 chaperones a diffusing DMC1-ssDNA complex to survey dsDNA for homology recognition during meiotic recombination.

Bingkai Cheng, Yanan Li, Yi Zhao, Yuting Zhang, Xia Zhang, Lishuang Chen, Hao Yang, Xiaoxuan Song, Zhiyun Ren, Cong Liu and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Bingkai ChengSchool of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
Yanan LiSchool of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
Yi ZhaoSchool of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
Yuting ZhangSchool of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
Xia ZhangSchool of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
Lishuang ChenSchool of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
Hao YangSchool of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Xiaoxuan SongSchool of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
Zhiyun RenSchool of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
Cong LiuInterdisciplinary Research Center on Biology and Chemistry, State Key Laboratory of Chemical Biology, Shanghai Academy of Natural Sciences, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 201210, China.ORCID 0000-0003-3425-6672
Jingdong XueDepartment of Biochemistry and Molecular Cell Biology, Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Shanghai Key Laboratory for Tumor Microenvironment and Inflammation, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.ORCID 0009-0008-7740-1589
Bing LiDepartment of Biochemistry and Molecular Cell Biology, Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Shanghai Key Laboratory for Tumor Microenvironment and Inflammation, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
Chao LiuGuangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623, China.
Wei LiGuangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623, China.ORCID 0000-0002-6235-0749
Bo SunSchool of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.ORCID 0000-0002-4590-7795

Funding

national nature science foundation of china 32271505national nature science foundation of china 32471508
6 · The paper itself

Abstract

Meiotic recombination ensures genetic diversity and accurate chromosome segregation by mediating reciprocal DNA exchange between homologous chromosomes. In this process, the meiosis-specific recombinase DMC1 plays a pivotal role in homology search and pairing, but the molecular mechanisms underlying its function remain unclear. Using single-molecule imaging, we demonstrate that the human DMC1-ssDNA presynaptic complex employs a diffusion-based mechanism to search for homologous DNA. Although this diffusing complex generates a migrating DNA "bubble," it cannot align with the homologous sequence in the absence of free DMC1 protein. Strikingly, the meiosis-specific cofactor complex HOP2-MND1 compensates for the lack of free DMC1 and enables homology recognition. Notably, HOP2-MND1 achieves this by codiffusing with the presynaptic complex, acting to clamp the ssDNA-dsDNA junctions and maintain an expanded DNA bubble conducive to sequence alignment. Our findings identify DMC1 together with HOP2-MND1 as a functional homology search unit and provide mechanistic insights into how auxiliary factors regulate DMC1-driven strand exchange during meiotic recombination.

Indexed as

DMC1HOP2–MND1meiotic recombinationrecombinasesingle-molecule

Identifiers

PMID41746729
PMCPMC12956812

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.