ArticlePlant cell reports2026
Visualizing application of RUBY-MAT system in maize genetic transformation and gene expression pattern detection.
Article in Plant cell reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
- A RUBY-Assisted Hairy Root Transformation Platform for Metabolomic Profiling and Preliminary Functional Analysis ofPlants (Basel, Switzerland) · 2026Article
- A novel positive selection system for plant transformation based on microbial biuret hydrolase and biuret.PloS one · 2026Article
Corrections and comments
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Authors and funding
15 authors.
Funding
Abstract
key messageRUBY-MAT system, integrating the RUBY reporter and MAT module, was innovatively applied to Agrobacterium-mediated maize transformation, and precise spatiotemporal gene expression analysis. Maize genetic improvement relies on efficient genetic transformation and gene expression monitoring technologies. In this study, we provided RUBY-MAT system with RUBY reporter and MAT (morphogene-assisted transformation), and realized its visual application in Agrobacterium-mediated genetic transformation of maize immature embryos and gene expression patterns. Experimental results demonstrated that RUBY-MAT system can be successfully applied in Agrobacterium-mediated genetic transformation of maize immature embryos, and RUBY reporter enables non-invasive monitoring of positive transformation events, as early as 7 days after culture, through the clearly distinguishable betalain of positive callus. Dynamic expression monitoring further revealed that RUBY reporter can reflect the spatiotemporal expression patterns of specific promoters in real time: in constitutively expressed RUBY plants, high activity was observed in tissues including tassels, anthers, and stem nodes; in ZmH1A promoter-driven RUBY lines, betalain accumulation clearly indicated gene expression in vascular bundles and stomatal guard cells; while in lines harboring the putative young leaf-specific ZmGLP1 promoter, betalain specifically accumulated in young leaves. Quantitative analysis confirmed a strong linear correlation between betalain absorbance and promoter activity, establishing the RUBY reporter as a reliable quantitative tool for monitoring transformation efficiency and gene expression at tissue and cellular levels. Collectively, the RUBY-MAT system offers robust technical support for maize genetic transformation and functional gene analysis, demonstrating broad application potential in both field and laboratory studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.