Evidence map›Paper›PMID 41746213›Full record

ArticleJournal of clinical microbiology2026

Rapid and reliable diagnosis of mucormycosis using colorimetric loop-mediated isothermal amplification.

Yiyou Gu, Belal A Ibrahim, Teclegiorgis Gebremariam, Sondus Alkhazraji, Robina Aerts, Yuri Vanbiervliet, Katrien Lagrou, Johan Maertens, Jürgen Prattes, Matthias Egger and 4 more

Abstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of clinical microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Yiyou GuThe Lundquist Institute at Harbor-University of California Los Angeles (UCLA) Medical Center, Torrance, California, USA.ORCID 0000-0002-8295-7222
Belal A IbrahimVitalex Biosciences LLC, Trabuco Canyon, California, USA.
Teclegiorgis GebremariamThe Lundquist Institute at Harbor-University of California Los Angeles (UCLA) Medical Center, Torrance, California, USA.
Sondus AlkhazrajiThe Lundquist Institute at Harbor-University of California Los Angeles (UCLA) Medical Center, Torrance, California, USA.
Robina AertsDepartment of Internal Medicine, University Hospitals Leuven, Leuven, Belgium.
Yuri VanbiervlietDepartment of Internal Medicine, University Hospitals Leuven, Leuven, Belgium.
Katrien LagrouDepartment of Microbiology Immunology and Transplantation, KU Leuven, Leuven, Belgium.ORCID 0000-0001-8668-1350
Johan MaertensDepartment of Microbiology Immunology and Transplantation, KU Leuven, Leuven, Belgium.
Jürgen PrattesDivision of Infectious Diseases, Department of Medicine, Translational Mycology Research Center, Excellence Centre for Medical Mycology (ECMM), Medical University of Graz, Graz, Austria.
Matthias EggerDivision of Infectious Diseases, Department of Medicine, Translational Mycology Research Center, Excellence Centre for Medical Mycology (ECMM), Medical University of Graz, Graz, Austria.
Karl DichtlDiagnostic and Research Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, Graz, Austria.
Jeffrey D JenksDurham County Department of Public Health, Durham, North Carolina, USA.
Martin HoeniglDivision of Infectious Diseases, Department of Medicine, Translational Mycology Research Center, Excellence Centre for Medical Mycology (ECMM), Medical University of Graz, Graz, Austria.ORCID 0000-0002-1653-2824
Ashraf S IbrahimThe Lundquist Institute at Harbor-University of California Los Angeles (UCLA) Medical Center, Torrance, California, USA.ORCID 0000-0003-3787-8530

Funding

UCLA Clinical Translational Science InstituteUL1TR001881 · NCATS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ARLEEN F. BROWN, ARASH NAEIM · 2016 to 2026
$118.1M
Novel Toxins and Receptors in Mucormycosis Pathogenesis and TreatmentR01AI063503 · NIAID · LUNDQUIST INSTITUTE FOR BIOMEDICAL INNOVATION AT HARBOR-UCLA MEDICAL CENTER · PI IBRAHIM, ASHRAF S. · 2006 to 2025
$7.3M
Project 3P01AI186818 · NIAID · LUNDQUIST INSTITUTE FOR BIOMEDICAL INNOVATION AT HARBOR-UCLA MEDICAL CENTER · PI DIMITRIOS P KONTOYIANNIS · 2025 to 2026
$6.7M
National Institute of Allergy and Infectious Diseases P01 AI186818-01NCATS NIH HHS UL1 TR001881NCATS NIH HHS UL1TR001881-01NIAID NIH HHS P01 AI186818NIAID NIH HHS R01 AI063503
6 · The paper itself

Abstract

Current diagnostic approaches for mucormycosis are often limited by low sensitivity and prolonged turnaround times, which result in delayed treatment and poor clinical outcomes. We developed a novel diagnostic method utilizing a colorimetric loop-mediated isothermal amplification (LAMP) assay for the rapid and sensitive detection of mucormycosis. The assay incorporates specifically designed primers capable of detecting as low as 0.001 picograms (pg) of spiked genomic DNA from Mucorales fungi. This LAMP assay demonstrated a high sensitivity of 98% and a 100% specificity of detecting fungal ribosomal DNA (rDNA) in bronchoalveolar lavage (BAL) samples collected from mice infected with Mucorales fungi (

Indexed as

ColorimetryMolecular Diagnostic TechniquesMucoralesMucormycosisNucleic Acid Amplification TechniquesAnimalsAntifungal AgentsBronchoalveolar Lavage FluidDisease Models, AnimalDNA, FungalDNA PrimersFemaleHumansMiceRapid Diagnostic TestsSensitivity and SpecificityAntifungal AgentsDNA, FungalDNA PrimersBALdiagnosisLAMPmucormycosisRhizopus

Identifiers

PMID41746213
PMCPMC13059791

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.