Sensitive Visual Detection of Breast Cancer Cells via a Dual-Receptor (Aptamer/Antibody) Lateral Flow Biosensor.

Yurui Zhou, Jiahui Wang, Ying Han, Meijing Ma, Junhong Li, Haidong Li, Xueji Zhang, Guodong Liu

Abstract read

In one paragraph

Article in Biosensors, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yurui ZhouSchool of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, China.

Jiahui WangSchool of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, China.

Ying HanSchool of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, China.

Meijing MaSchool of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, China.

Junhong LiSchool of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, China.

Haidong LiSchool of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, China.ORCID 0009-0005-8533-7319

Xueji ZhangMarshall Laboratory of Biomedical Engineering, Research Center for Biosensor and Nanotheranostic, School of Biomedical Engineering, Shenzhen University, Shenzhen 518060, China.

Guodong LiuSchool of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, China.ORCID 0000-0003-1063-208X

Funding

Startup Foundation for Advanced Talents of Linyi University Z6122047the National Key Research and Development Program of the Ministry of Science and Technology 2022YFB3207200

6 · The paper itself

Abstract

We report a novel dual-receptor lateral flow biosensor (LFB) for the rapid, sensitive, and visual detection of MCF-7 breast cancer cells as a model for circulating tumor cells (CTCs). The biosensor employs a MUC1-specific aptamer conjugated to colloidal gold nanoparticles as the detection probe and an anti-MUC1 antibody immobilized at the test line as the capture probe, forming a unique "aptamer-cell-antibody" sandwich complex upon target recognition. This design enables instrument-free, visual readout within minutes, achieving a detection limit of 675 cells. The assay also demonstrates robust performance in spiked human blood samples, highlighting its potential as a simple, cost-effective dual-mode point-of-care testing (POCT) platform. This platform supports both rapid visual screening and optional strip-reader-based quantification, making it suitable for early detection and monitoring of breast cancer CTCs.

Indexed as

Aptamers, NucleotideBiosensing TechniquesBreast NeoplasmsNeoplastic Cells, CirculatingFemaleGoldHumansMCF-7 CellsMetal NanoparticlesMucin-1Aptamers, NucleotideGoldMucin-1aptamer–antibody sandwich assaylateral flow biosensorMCF-7 cellspoint-of-care testing (POCT)visual detection

Identifiers

PMID41744704

DOI10.3390/bios16020085

PMCPMC12938399

What OpenQuestion holds

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LicenceCC BY

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.