ArticleBiology2026
Assessment of Freshwater Unionidae Using Environmental DNA Metabarcoding in Lentic Ecosystems: Implications for Spatial Sampling Strategies.
Article in Biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Environmental DNA (eDNA) metabarcoding has become a powerful, non-invasive method for detecting aquatic organisms. However, optimal sampling strategies for benthic taxa in lentic ecosystems remain unclear. This study evaluated the effectiveness of eDNA metabarcoding in detecting freshwater Unionidae mussels in lake water columns and examined their spatial and seasonal distribution patterns. We validated a mini-barcode primer targeting the mitochondrial 16S rDNA of unionid mussels through controlled laboratory experiments and field tests, confirming reliable amplification and accurate taxonomic assignment of freshwater bivalve DNA. Field surveys were conducted in four lakes within the Nakdong River basin, where eDNA samples were collected from littoral zones and from surface, mid-, and bottom layers of central lake areas during autumn and winter. Metabarcoding analysis identified 79 amplicon sequence variants (ASVs) representing four unionid taxa, with
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