Evidence map›Paper›PMID 41742634›Full record

ArticleAngewandte Chemie (International ed. in English)2026

Controlled Delivery and Light-Induced Release of Magic Spot Nucleotides in Escherichia coli.

Christoph Popp, Patrick Moser, Anselm Schwoerbel, Xinwei Liu, Johannes Freitag, Pinku Sarmah, Isabel Prucker, Robert Zscherp, Xuan Wang, Philipp Klahn and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Angewandte Chemie (International ed. in English), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Christoph PoppFaculty of Chemistry and Pharmacy, Institute of Organic Chemistry & CIBSS, Centre For Integrative Biological Signalling Studies, Albert-Ludwigs University Freiburg, Freiburg, Germany.
Patrick MoserFaculty of Chemistry and Pharmacy, Institute of Organic Chemistry & CIBSS, Centre For Integrative Biological Signalling Studies, Albert-Ludwigs University Freiburg, Freiburg, Germany.
Anselm SchwoerbelFaculty of Chemistry and Pharmacy, Institute of Organic Chemistry & CIBSS, Centre For Integrative Biological Signalling Studies, Albert-Ludwigs University Freiburg, Freiburg, Germany.
Xinwei LiuCenter For Synthetic Microbiology (SYNMIKRO), Marburg University, Marburg, Germany.
Johannes FreitagCenter For Synthetic Microbiology (SYNMIKRO), Marburg University, Marburg, Germany.
Pinku SarmahFaculty of Medicine, Institute For Biochemistry and Molecular Biology, ZBMZ, Albert-Ludwigs University Freiburg, Freiburg, Germany.
Isabel PruckerFaculty of Chemistry and Pharmacy, Institute of Organic Chemistry & CIBSS, Centre For Integrative Biological Signalling Studies, Albert-Ludwigs University Freiburg, Freiburg, Germany.
Robert ZscherpDepartment of Chemistry and Molecular Biology, Division of Organic and Medicinal Chemistry, University of Gothenburg, Gothenburg, Sweden.
Xuan WangFaculty of Chemistry and Pharmacy, Institute of Organic Chemistry & CIBSS, Centre For Integrative Biological Signalling Studies, Albert-Ludwigs University Freiburg, Freiburg, Germany.
Philipp KlahnDepartment of Chemistry and Molecular Biology, Division of Organic and Medicinal Chemistry, University of Gothenburg, Gothenburg, Sweden.
Hans-Georg KochFaculty of Medicine, Institute For Biochemistry and Molecular Biology, ZBMZ, Albert-Ludwigs University Freiburg, Freiburg, Germany.
Gert BangeCenter For Synthetic Microbiology (SYNMIKRO), Marburg University, Marburg, Germany.
Henning J JessenFaculty of Chemistry and Pharmacy, Institute of Organic Chemistry & CIBSS, Centre For Integrative Biological Signalling Studies, Albert-Ludwigs University Freiburg, Freiburg, Germany.ORCID 0000-0002-1025-9484

Funding

Deutsche Forschungsgemeinschaft 317784314Deutsche Forschungsgemeinschaft 390939984Deutsche Forschungsgemeinschaft 403222702European Research Council 864246Medical Faculty, University of Freiburg 2023/B3-Fol
6 · The paper itself

Abstract

The "magic spot" nucleotides (MSNs) ppGpp and pppGpp constitute bacterial alarmones that orchestrate the conserved stringent response, a global regulatory mechanism enabling bacteria to adapt to nutrient deprivation and other environmental stresses. Current strategies to manipulate MSN levels rely mainly on genetic or environmental approaches, which are slow and lack temporal control. Chemical tools such as photocaged MSN analogues could provide such temporal control over MSN levels. However, the high negative charge of MSNs prevents spontaneous passage through the complex bacterial cell envelope. Here, we report the synthesis of photocaged, clickable, and isotope-labeled MSN analogues and their delivery into Escherichia coli comparing different approaches. A cyclodextrin-based synthetic nucleotide transporter facilitated uptake. Upon 400 nm irradiation, these probes were photo-released inside living cells, where we tracked their conversion from pppGpp to ppGpp by capillary electrophoresis mass spectrometry and demonstrated their ability to alter growth in a (p)ppGpp

Indexed as

Escherichia coliGuanosine PentaphosphateGuanosine TetraphosphateLightNucleotidesGuanosine PentaphosphateGuanosine TetraphosphateNucleotidesCellular deliverymagic spot nucleotidesphotocagesppGppstringent response

Identifiers

PMID41742634
PMCPMC13053917

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.