Protocol for quantifying vertebral column morphology for the statistical analysis of scoliosis severity in zebrafish.

Brittney Voigt, Ece Atayeter, Ron Sistrunk, Ryan S Gray

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In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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4 · The record

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5 · Who and what money

Authors and funding

4 authors.

Brittney VoigtDepartment of Nutritional Sciences, The University of Texas at Austin, Austin, TX 78705, USA; Department of Pediatrics, Dell Pediatric Research Institute, The University of Texas at Austin, Austin, TX 78723, USA. Electronic address: brittney.voigt@austin.utexas.edu.

Ece AtayeterDepartment of Nutritional Sciences, The University of Texas at Austin, Austin, TX 78705, USA; Department of Pediatrics, Dell Pediatric Research Institute, The University of Texas at Austin, Austin, TX 78723, USA.

Ron SistrunkDepartment of Nutritional Sciences, The University of Texas at Austin, Austin, TX 78705, USA; Department of Pediatrics, Dell Pediatric Research Institute, The University of Texas at Austin, Austin, TX 78723, USA.

Ryan S GrayDepartment of Nutritional Sciences, The University of Texas at Austin, Austin, TX 78705, USA; Department of Pediatrics, Dell Pediatric Research Institute, The University of Texas at Austin, Austin, TX 78723, USA. Electronic address: ryan.gray@austin.utexas.edu.

Funding

Non-coding/epigenetic regulationP01HD084387 · NICHD · UT SOUTHWESTERN MEDICAL CENTER · PI Nadav Ahituv, LILIANNA SOLNICAKREZEL · 2016 to 2026

$14.2M

NICHD NIH HHS P01 HD084387

6 · The paper itself

Abstract

Here, we present a protocol for quantifying zebrafish vertebral column morphology using micro-computed tomography (microCT) datasets for scoliosis researchers. We describe steps for orientation of skeletal and cranial microCT datasets in DataViewer, vertebral position measurements in ImageJ/Fiji, and analysis of vertebral position in R. The approach described here graphically depicts vertebral position while providing a means to quantify scoliosis severity and perform statistical analysis of differences in vertebral column morphology in zebrafish. For complete details on the use and execution of this protocol, please refer to Voigt et al.

Indexed as

ScoliosisSpineX-Ray MicrotomographyZebrafishAnimalsDisease Models, AnimalDevelopmental biologyMicroscopyModel Organisms

Identifiers

PMID41742410

DOI10.1016/j.xpro.2026.104389

PMCPMC13023086

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LicenceCC BY-NC-ND

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.