Evidence map›Paper›PMID 41741580›Full record

ArticleScientific reports2026

DNA methylation at OsAmy3E promoter is involved in grain quality under heat stress in rice (Oryza sativa L.).

Wun-Jin Chen, Chetphilin Suriyasak, Hue Thi Nong, Yui Oyama, Takuya Kobayashi, Ryo Matsumoto, Ryusuke Kawaguchi, Xinyue Yang, Katsunori Miyahara, Norimitsu Hamaoka and 1 more

Abstract read
In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Wun-Jin Chen *Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, 819-0395, Japan.
Chetphilin Suriyasak *Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, 819-0395, Japan.
Hue Thi NongGraduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, 819-0395, Japan.
Yui OyamaGraduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, 819-0395, Japan.
Takuya KobayashiGraduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, 819-0395, Japan.
Ryo MatsumotoGraduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, 819-0395, Japan.
Ryusuke KawaguchiGraduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, 819-0395, Japan.
Xinyue YangGraduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, 819-0395, Japan.
Katsunori MiyaharaGraduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, 819-0395, Japan.
Norimitsu HamaokaTropical Crop and Environment Section, Institute of Tropical Agriculture, Kyushu University, Fukuoka, 819-0395, Japan.
Yushi IshibashiGraduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, 819-0395, Japan. yushi@agr.kyushu-u.ac.jp.

Funding

Japan Society for the Promotion of Science JP20H02971Japan Society for the Promotion of Science JP23K26889JST-Mirai Program JPMJMI21E3
6 · The paper itself

Abstract

High-temperature stress during grain filling significantly reduces rice grain yield and quality. The heat-tolerant cultivar Kumasannochikara (Kuma) has a lower chalky-grain percentage and better grain quality under heat stress than the heat-sensitive Hinohikari (Hino), owing to repression of the α-amylase gene OsAmy3E during grain filling. Here, we propose that the expression of OsAmy3E during grain filling under heat stress is epigenetically regulated, as the promoter region of OsAmy3E in Kuma is highly methylated. In developing Kuma × Hino F1 seed, hypermethylation of the OsAmy3E promoter suppressed its expression. Screening of DNA methylation levels in F2 plants divided plants into non-methylated and methylated groups. Methylated plants of both Hino × Kuma and Kuma × Hino had repressed OsAmy3E gene expression and better grain quality under heat stress than non-methylated plants. These findings suggest that the suppression of α-amylase gene expression through epigenetic regulation could improve rice grain quality under heat stress.

Indexed as

alpha-AmylasesDNA MethylationEdible GrainHeat-Shock ResponseOryzaPlant ProteinsPromoter Regions, GeneticEpigenesis, GeneticGene Expression Regulation, PlantHot TemperatureSeedsalpha-AmylasesPlant Proteins

Identifiers

PMID41741580
PMCPMC13044291

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.