Evidence map›Paper›PMID 41739395›Full record

ArticleCrop health2026

Highly specific and super-sensitive Dot-ELISA and colloidal gold immunochromatographic strips for the detection of Burkholderia glumae and Burkholderia plantarii of Rice bacterial panicle blight.

Jie Dong, Weijia Mao, Cui Zhang, Bin Li, Zhiyan An, Jinyan Luo, Jianxiang Wu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Crop health, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Jie DongState Key Laboratory of Rice Biology and Breeding, Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou, China.
Weijia MaoState Key Laboratory of Rice Biology and Breeding, Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou, China.
Cui ZhangState Key Laboratory of Rice Biology and Breeding, Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou, China.
Bin LiState Key Laboratory of Rice Biology and Breeding, Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou, China.
Zhiyan AnMangshi Plant Protection and Plant Quarantine Station, Mangshi, China. lxszbz@163.com.ORCID http://orcid.org/0009-0007-9849-4537
Jinyan LuoDepartment of Plant Quarantine, Shanghai Extension and Service Center of Agriculture Technology, Shanghai, China. toyanzi@126.com.ORCID http://orcid.org/0000-0001-7442-9158
Jianxiang WuState Key Laboratory of Rice Biology and Breeding, Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou, China. wujx@zju.edu.cn.ORCID http://orcid.org/0000-0002-7611-7833

Funding

Earmarked Fund for Modern Agro-industry Technology Research System nycytx-001Shanghai Agricultural Science and Technology Innovation Project T2023101Special Fund Project for Science and Technology Innovation Strategy of Guangdong Province ZDYF2022XDNY240
6 · The paper itself

Abstract

Burkholderia glumae (B. glumae) and Burkholderia plantarii (B. plantarii) are primary causal agents of rice bacterial panicle blight (RBPB) and cause substantial yield losses in rice worldwide. Given their seed-borne transmission characteristics, quarantine status and destructive hazards, rapid, super-sensitive, highly specific on-site detection technologies are urgently needed. Here, using B. glumae Os48 and B. plantarii ZJ171 as immunogens, we prepared two highly specific and ultra-sensitive monoclonal antibodies (mAbs) against B. glumae (4A7 and 8C5) and two highly specific and ultra-sensitive mAbs against B. plantarii (12B5 and 14B3). We then developed dot-enzyme-linked immunosorbent assays (Dot-ELISA) and colloidal gold immunochromatographic strip (CGICS) assays for detecting B. glumae and B. plantarii with the prepared mAbs as the detection antibodies. These developed mAb-based serological techniques enabled the rapid, broad-spectrum, and specific detection of B. glumae and B. plantarii, respectively, and showed no cross-reaction with other control plant bacteria included in the analysis. Moreover, the detection limits of the Dot-ELISAs for B. glumae or B. plantarii were up to 1.96 × 10

Indexed as

Burkholderia glumaeBurkholderia plantariiColloidal gold immunochromatographic strip assayDot-ELISAMonoclonal antibodyRice bacterial panicle blight

Identifiers

PMID41739395
PMCPMC12936288

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.