Evidence map›Paper›PMID 41739306›Full record

ArticleFood and environmental virology2026

Comparative Investigation and Trends of Respiratory Viruses Using Wastewater-Based Epidemiological Surveillance in Patras, Greece.

Zoi Anastopoulou, Rafail Fokas, Kalypso-Angeliki Koukouvini, Angeliki Chatziantoniou, Apostolos Vantarakis

Abstract readComparative Study
In one paragraph

Article in Food and environmental virology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Zoi AnastopoulouLaboratory of Public Health, Epidemiology and Quality of Life, School of Medicine, University of Patras, 26504, Patras, Greece.ORCID 0009-0007-2597-2446
Rafail FokasLaboratory of Public Health, Epidemiology and Quality of Life, School of Medicine, University of Patras, 26504, Patras, Greece.ORCID 0009-0006-8957-8029
Kalypso-Angeliki KoukouviniLaboratory of Public Health, Epidemiology and Quality of Life, School of Medicine, University of Patras, 26504, Patras, Greece.ORCID 0000-0001-8708-0960
Angeliki ChatziantoniouLaboratory of Public Health, Epidemiology and Quality of Life, School of Medicine, University of Patras, 26504, Patras, Greece.ORCID 0009-0007-3582-1705
Apostolos VantarakisLaboratory of Public Health, Epidemiology and Quality of Life, School of Medicine, University of Patras, 26504, Patras, Greece. avanta@upatras.gr.ORCID 0000-0003-4411-3739

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The COVID-19 pandemic has underscored the importance of alternative epidemiological tools capable of providing real-time, population-level insights into infectious disease dynamics. Wastewater-based epidemiology (WBE) has emerged as a powerful, non-invasive method for tracking viral transmission within communities. While initially focused on SARS-CoV-2, WBE now offers the potential for multiplex surveillance of a broader range of respiratory pathogens. This study applied WBE to monitor the circulation of six major respiratory viruses such as Human adenovirus (HAdV), SARS-CoV-2, Influenza A and B, Respiratory Syncytial Virus (RSV) A/B, and Rhinoviruses, over a six-month period (ISO weeks 2022-40 to 2023-13) in the city of Patras, Greece. Weekly composite samples from a central wastewater treatment plant were analyzed via quantitative PCR, and viral genome concentrations were normalized per 100,000 inhabitants. The results revealed distinct circulation patterns: HAdV and SARS-CoV-2 were detected consistently throughout the period, while Influenza A peaked during the winter months, followed by Influenza B. RSV, however, appeared earlier in the season (first detectable from ISO week 2022-46) and overlapped with Influenza. Rhinoviruses displayed intermittent peaks, indicating multiple waves or persistent low-level transmission. Correlation analyses showed strong positive associations between influenza viruses, RSV, and SARS-CoV-2, suggesting synchronized seasonal trends. Hierarchical cluster analysis classified the viruses into three distinct groups: (1) an epidemic cluster including Influenza A/B, RSV, and Rhinoviruses; (2) a persistently present cluster represented by HAdV; and (3) a separate episodic cluster characterized by SARS-CoV-2. These groupings reflect differences in viral epidemiology and shedding behaviour. This study confirms the effectiveness of WBE in tracking the temporal dynamics of multiple respiratory viruses and provides evidence of its utility in supplementing traditional clinical surveillance systems. The consistent detection of underreported pathogens such as HAdV underscores the added value of environmental monitoring for public health preparedness and early warning applications. These findings advocate for the integration of WBE into routine respiratory virus surveillance frameworks.

Indexed as

COVID-19Respiratory Tract InfectionsWastewaterWastewater-Based Epidemiological MonitoringGreeceHumansInfluenza A virusRespiratory Syncytial Virus, HumanRhinovirusSARS-CoV-2SeasonsWastewater

Identifiers

PMID41739306
PMCPMC12935714

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.