ArticleDrug design, development and therapy2026
Preclinical Assessment of HLA-A*02:01-Restricted PSMA and STEAP1 Epitopes for Peptide-Based Immunotherapy in Prostate Cancer.
Article in Drug design, development and therapy, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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11 authors.
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Abstract
Background: Peptide-based immunotherapy targeting tumor-associated antigens presents a promising approach for prostate cancer intervention. In this study, we employed a preclinical approach to evaluate immunogenic epitope candidates derived from STEAP1 and PSMA, two well-characterized prostate cancer-associated antigens. Methods: High-affinity cytotoxic T lymphocyte (CTL) epitopes restricted to HLA-A*02:01 were predicted and evaluated for antigenicity, conservation, and proteasomal processing. Molecular docking with HLA-A*02:01 was performed and peptides were synthesized for experimental validation. In-vitro assays including ELISA-based MHC binding, IFN-γ ELISpot, and intracellular cytokine staining (ICS) were conducted using splenocytes isolated from HLA-A*02:01 transgenic mice. Results: Four peptides (P1, P2, P3 and P4), demonstrated strong binding to HLA-A*02:01 in-silico and were structurally compatible with the MHC class I groove. ELISA confirmed high binding for P3 and P1 at lower concentrations, while P2 and P4 showed moderate affinity. ELISpot assays showed robust IFN-γ responses in peptide-stimulated splenocytes, particularly for P3 and P1. ICS confirmed CD8⁺ T cell activation and polyfunctional cytokine expression. A comparative Nano-immunogenicity profile revealed P3 as the most potent candidate. Conclusion: This study provides preclinical evidence of antigenicity and MHC-I compatibility of four prostate cancer-derived CTL epitopes using a transgenic mouse model. These findings support the advancement of these peptides as candidates for peptide-based immunotherapy in prostate cancer.
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