ArticleThe FEBS journal2026
Unveiling the influence of salt concentration on the different stages of the catalytic cycle of a halophilic enzyme.
Article in The FEBS journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Enzymes from halophilic organisms function in near-saturating salt concentrations. To explore enzyme adaptation to these conditions and the broader effects of salt on enzyme catalysis, we examined the salt effect on the kinetics of a glucose-6-phosphate dehydrogenase from the halophilic archaeon Haloferax volcanii (HvG6PDH). This enzyme has a negatively charged surface and catalyzes the oxidation of the negatively charged substrate glucose-6-phosphate (G6PDH activity) and glucose (glcDH activity). We found strikingly different salt effects on the two activities. Steady-state kinetics experiments revealed a 50-fold decrease in K
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