Evidence map›Paper›PMID 41736107›Full record

ArticleJournal of nanobiotechnology2026

Programmable trivalent nanocage vaccine confers durable cross-species protection against Bordetella bronchiseptica infection.

Wenna He, Lingli Wang, Xiaoyu Zhang, Jiawu Wan, Feiyu Chen, Zeheng Ren, Qinghua Shang, Zhiyong Song, Zhen F Fu, Ling Zhao and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of nanobiotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Wenna HeThe National Key Laboratory of Agricultural Microbiology, The Hubei Hongshan Laboratory, The Key Laboratory of Preventive Veterinary Medicine of Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Lingli WangThe National Key Laboratory of Agricultural Microbiology, The Hubei Hongshan Laboratory, The Key Laboratory of Preventive Veterinary Medicine of Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Xiaoyu ZhangThe National Key Laboratory of Agricultural Microbiology, The Hubei Hongshan Laboratory, The Key Laboratory of Preventive Veterinary Medicine of Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Jiawu WanThe National Key Laboratory of Agricultural Microbiology, The Hubei Hongshan Laboratory, The Key Laboratory of Preventive Veterinary Medicine of Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Feiyu ChenThe National Key Laboratory of Agricultural Microbiology, The Hubei Hongshan Laboratory, The Key Laboratory of Preventive Veterinary Medicine of Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Zeheng RenThe National Key Laboratory of Agricultural Microbiology, The Hubei Hongshan Laboratory, The Key Laboratory of Preventive Veterinary Medicine of Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Qinghua ShangThe National Key Laboratory of Agricultural Microbiology, The Hubei Hongshan Laboratory, The Key Laboratory of Preventive Veterinary Medicine of Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Zhiyong SongThe National Key Laboratory of Agricultural Microbiology, College of Science, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Zhen F FuThe National Key Laboratory of Agricultural Microbiology, The Hubei Hongshan Laboratory, The Key Laboratory of Preventive Veterinary Medicine of Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Ling ZhaoThe National Key Laboratory of Agricultural Microbiology, The Hubei Hongshan Laboratory, The Key Laboratory of Preventive Veterinary Medicine of Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, China. zling604@outlook.com.
Ming ZhouThe National Key Laboratory of Agricultural Microbiology, The Hubei Hongshan Laboratory, The Key Laboratory of Preventive Veterinary Medicine of Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, China. mingzhou@hzau.edu.cn.

Funding

the Fundamental Research Funds for the Central Universities 2662023PY005the National Key Research and Development Program of China 2022YFD1800100
6 · The paper itself

Abstract

Bordetella bronchiseptica (B. bronchiseptica), a respiratory pathogen endemic in companion animals, poses an escalating zoonotic threat to humans due to intensified human-pet contact, particularly among immunocompromised individuals. Here, we report a programmable trivalent nanocage vaccine, mi3-F1P2Fim2, featuring immunodominant regions of FHA, Prn, and Fim2 covalently fused into a single-chain construct and displayed on a self-assembling mi3 nanocage via the SpyTag003/SpyCatcher003 system, enabling high-density and multivalent antigen presentation. Compared with its soluble monomeric counterpart, mi3-F1P2Fim2 induced significantly enhanced humoral and cellular immune responses, characterized by a 1.98-fold elevation in antigen-specific IgG titers, improved IgG1/IgG2a subclass balance, and heightened dendritic cell activation. The vaccine facilitated efficient uptake by antigen-presenting cells and induced robust germinal center reactions. Notably, mi3-F1P2Fim2 conferred complete protection against B. bronchiseptica challenge in both murine and canine models, achieving > 1000-fold reduction in pulmonary bacterial burden with concomitant alleviation of lung pathology. These data demonstrate that mi3-F1P2Fim2 confers potent, cross-species protective immunity against B. bronchiseptica, establishing it as a promising vaccine candidate for preventing zoonotic respiratory infections. By targeting this clinically significant but understudied pathogen, our findings advance strategies to disrupt B. bronchiseptica transmission and enable targeted immunization aligned with One Health objectives.

Indexed as

Bacterial VaccinesBordetella bronchisepticaBordetella InfectionsNanovaccinesAnimalsAntibodies, BacterialAntigens, BacterialBone MarrowCell LineDendritic CellsDogsFemaleGerminal CenterImmunity, CellularImmunoglobulin GMiceAntibodies, BacterialAntigens, BacterialBacterial VaccinesImmunoglobulin GNanovaccinesBordetella bronchisepticaIncreased antigen uptakemi3 nanocagesRobust germinal center reactionsTrivalent nanocage vaccine

Identifiers

PMID41736107
PMCPMC13041014

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.