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ArticleMolecular biology reports2026

First report of apple stem grooving virus infecting a species of Lilium (Lilium brownii) in China.

Yang Shen, Qundan Lv, Guokai Xia, Fang Ye, Zhoufo Lu, Zhanqi Wang, Xueting Zhong

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Article in Molecular biology reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

7 authors.

Yang Shen *College of Life Sciences, Huzhou University, Huzhou, 313000, China.
Qundan Lv *Lishui Institute of Agriculture and Forestry Sciences, Lishui, 323020, China.
Guokai XiaCollege of Life Sciences, Huzhou University, Huzhou, 313000, China.
Fang YeCollege of Life Sciences, Huzhou University, Huzhou, 313000, China.
Zhoufo LuCollege of Life Sciences, Huzhou University, Huzhou, 313000, China.
Zhanqi WangCollege of Life Sciences, Huzhou University, Huzhou, 313000, China. zhqwang@zju.edu.cn.
Xueting ZhongCollege of Life Sciences, Huzhou University, Huzhou, 313000, China. zxt@zjhu.edu.cn.

Funding

Huzhou Public Welfare Application Research Project of China 2024GZ34National Natural Science Foundation of China 32102162
6 · The paper itself

Abstract

backgroundLily (Lilium brownii), a perennial bulbous herbaceous plant in the Lilium genus of the Liliaceae family, is esteemed in traditional Chinese medicine and utilized as a functional food due to the high nutritional content of its bulbs. However, the cultivation of lilies is severely constrained by viral infections, which can cause substantial yield losses and markedly compromise both commercial production and medicinal value. Virus disease has become a significant threat to lily production worldwide and has occurred in several provinces of China. METHODS AND

resultsIn this study, a new isolate of apple stem grooving virus (ASGV-LS) (Capillovirus mali) from L. brownii in Lishui City, Zhejiang province, China was first identified using the next-generation sequencing (NGS) technique combined with reverse transcription PCR and 3'/5'-rapid amplification of cDNA ends methods. ASGV-LS possessed a positive-sense RNA genome with a full-length sequence of 6469 nucleotides (nt), including a 27-nt poly(A) tail and two overlapping open reading frames (ORFs). ORF1 encoded a 241.0-kDa polyprotein, whereas ORF2 encoded a 36.1-kDa movement protein. Sequence analysis revealed that ASGV-LS had the highest nucleotide sequence identity of 94.5% with ASGV-HZ (GenBank: MW259059) isolated from Gynura japonica. Further phylogenetic analysis of the complete genome sequences indicated that ASGV-LS clusters into a subclade with ASGV-HZ, ASGV-PG (GenBank: MK427058), ASGV-ZG2 (GenBank: MN495979), and ASGV-KFP (GenBank: KR106996), which belong to the genus Capillovirus.

conclusionThese findings establish ASGV-LS is a new isolate of the ASGV species of the genus Capillovirus in the family Betaflexiviridae. To our knowledge, this is the first report of L. brownii being infected by ASGV in China.

Indexed as

FlexiviridaeLiliumChinaGenome, ViralHigh-Throughput Nucleotide SequencingOpen Reading FramesPhylogenyPlant DiseasesASGVGenome sequenceLilium browniiNGSPhylogenetic analysis

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