ArticleJournal of oral biology and craniofacial research
Effect of type I collagen on TLR-3 induced MMP-13 expression in human periodontal ligament fibroblasts.
Article in Journal of oral biology and craniofacial research. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Letter to the Editor regarding "Effect of type I collagen on TLR-3 induced MMP-13 expression in human periodontal ligament fibroblasts".Journal of oral biology and craniofacial researchArticle
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: This study examined the effects of type I collagen, alone and in combination with poly I:C-a toll-like receptor 3 (TLR3) agonist-on matrix metalloproteinase-13 (MMP-13) expression and wound healing in human periodontal ligament (hPDL) fibroblasts. Methods: hPDL fibroblasts were cultured and divided into four treatment groups: control, type I collagen (50 μg/mL), poly I:C (10 μg/mL), and a combination of type I collagen with poly I:C. Cytotoxicity was evaluated using the MTT assay. Cell migration was assessed via scratch assay at 0, 24, and 48 h. MMP-13 expression was quantified at both the mRNA and protein levels by real-time PCR and ELISA, respectively. Results: After 24 h, the MTT assay indicated that none of the four treatment groups exhibited cytotoxicity toward hPDL fibroblasts. In the scratch assay at 24 and 48 h, type I collagen group demonstrated the fastest wound closure, whereas the poly I:C group showed the slowest migration. Regarding MMP-13 expression, the combination group displayed the highest mRNA levels, while ELISA revealed that both the combination and poly I:C groups had the greatest protein expression relative to the control. Conclusion: This study provides evidence for an interaction between extracellular matrix signals and innate immune activation. Type I collagen promoted hPDL fibroblast migration, whereas poly I:C-a TLR3 agonist-upregulated MMP-13 expression, with the greatest effect observed in combination with collagen.
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