Evidence map›Paper›PMID 41732287›Full record

ArticleVeterinary and animal science2026

Field performance of a point-of-care PCR platform for the detection of influenza A virus in growing pigs.

Everett W Dalesio, Ting-Yu Cheng, Andrew S Bowman, Sylvester Ochwo, Rachel A Schambow, Maria Sol Pérez, Andres Perez, Andréia G Arruda

Abstract read
In one paragraph

Article in Veterinary and animal science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Everett W DalesioDepartment of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, OH, United States.
Ting-Yu ChengDepartment of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, OH, United States.
Andrew S BowmanDepartment of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, OH, United States.
Sylvester OchwoDepartment of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, United States.
Rachel A SchambowDepartment of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, United States.
Maria Sol PérezDepartment of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, United States.
Andres PerezDepartment of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, United States.
Andréia G ArrudaDepartment of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, OH, United States.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Point-of-care (POC) testing involves conducting diagnostic assays near patients to enable rapid decision-making and timely intervention. To date, evaluation of POC devices under field conditions in veterinary medicine has been limited. The objective of this study was to describe the use and performance of a POC system in the field and with varying sample conditions using influenza A virus in swine. Seventy oral fluid samples were collected from ten swine farms and aliquoted into five treatments: portable PCR performed on farm (FARM), while driving (RIDE), after 24 h incubation at room temperature (RT), on ice (ICE), and as a gold standard, samples were also submitted for testing at the Ohio Animal Disease Diagnostic Laboratory (LAB). Influenza A virus was detected at least in one oral fluid sample for six out of the ten farms sampled according to gold standard results; within-herd prevalence in positive farms varied from 16.7 to 100.0%. POC system's Ct values were statistically associated with the gold standard results. POC tests showed high specificity, with median estimates ranging from 0.954 to 0.958 and 95% credible intervals (CrIs) consistently above 0.91. Sensitivity of POC tests varied more substantially, with the lowest observed in the ICE setting (median 0.805; 95% CrI: 0.607-0.945) and the highest in the RT setting (median 0.926; 95% CrI: 0.766-0.995). Our study showed that the POC PCR system was a promising method for on-site diagnostic testing, providing results consistent with official laboratory results.

Indexed as

Point-of-carePortable PCRSwine influenza

Identifiers

PMID41732287
PMCPMC12924748

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.