Evidence map›Paper›PMID 41731527›Full record

ArticleStem cell research & therapy2026

m

Qiaonan Ye, Qianke Tao, Xuemei Long, Fangzhi Lou, Jingxiang Li, Xiaobing Li, Dan Tan, Jingang Xiao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Qiaonan Ye *Department of Plastic and Burn Surgery, The Affiliated Hospital, Southwest Medical University, Luzhou, 646000, China.
Qianke Tao *Department of Plastic and Burn Surgery, The Affiliated Hospital, Southwest Medical University, Luzhou, 646000, China.
Xuemei Long *Luzhou Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, The Affiliated Stomatological Hospital, Southwest Medical University, Luzhou, 646000, China.
Fangzhi LouLuzhou Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, The Affiliated Stomatological Hospital, Southwest Medical University, Luzhou, 646000, China.
Jingxiang LiLuzhou Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, The Affiliated Stomatological Hospital, Southwest Medical University, Luzhou, 646000, China.
Xiaobing LiDepartment of Plastic and Burn Surgery, The Affiliated Hospital, Southwest Medical University, Luzhou, 646000, China.
Dan TanLuzhou Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, The Affiliated Stomatological Hospital, Southwest Medical University, Luzhou, 646000, China. tandan@swmu.edu.cn.ORCID http://orcid.org/0000-0001-9660-181X
Jingang XiaoDepartment of Plastic and Burn Surgery, The Affiliated Hospital, Southwest Medical University, Luzhou, 646000, China. xiaojingang@swmu.edu.cn.ORCID http://orcid.org/0000-0003-1300-7191

Funding

2023 Key Project of The Affiliated Stomatological Hospital of Southwest Medical University 2023Z02National Natural Science Foundation of China 82370938Open Project of State Key Laboratory of Oral Diseases SKLOD2025OF05Project of Science & Technology Department of Sichuan Province 2024NSFSC2087Science and Technology Project of Health Commission of Sichuan Province 24WXXT11Scientific and Technological Project of Luzhou 2025MYF002Scientific and Technological Project of Luzhou 2025MYF024The Youth Innovation Project of Sichuan Medical Association Q20250047
6 · The paper itself

Abstract

backgroundImpaired osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) contributes to osteoporosis (OP) pathogenesis. While RNA modifications such as N6-methyladenosine (m

methodsFemale Sprague-Dawley rats were subjected to ovariectomy (OVX) to establish an OP model. BMSCs were isolated, characterized by flow cytometry and trilineage differentiation, and subjected to osteogenic induction. Bhlhe22 expression was modulated via lentiviral overexpression, siRNA-mediated knockdown, and adeno-associated virus (AAV)-mediated shRNA knockdown in vivo. Gene expression was assessed by immunofluorescence, quantitative PCR (qPCR), western blotting, and RNA sequencing. Osteogenic differentiation was evaluated via alkaline phosphatase (ALP), alizarin red S (ARS) staining, and osteogenic marker expression. Micro-computed tomography (micro-CT) and histology were used to assess bone structure. Mechanisms were investigated via PI3K-Akt pathway activation (recilisib), chromatin immunoprecipitation sequencing (ChIP-seq), ChIP-qPCR, dot blot, methylated RNA immunoprecipitation (MeRIP)-qPCR, luciferase reporter assays, and site-directed mutagenesis.

resultsGlobal m

conclusionsm

Indexed as

AdenosineGlycogen Synthase Kinase 3 betaOsteogenesisOsteoporosisPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktAnimalsCell DifferentiationFemaleMesenchymal Stem CellsRatsRats, Sprague-DawleyRNA MethylationSignal TransductionAdenosineGlycogen Synthase Kinase 3 betaN-methyladenosinePhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktBhlhe22BMSCsN6-methyladenosineOsteogenesisOsteoporosisPI3K-AKT-GSK3β axis

Identifiers

PMID41731527
PMCPMC13037282

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.