Evidence map›Paper›PMID 41731164›Full record

ArticleWorld journal of microbiology & biotechnology2026

Heterologous expression, purification and functional characterization of recombinant serratiopeptidase from Serratia marcescens AD-W2.

Devtulya Chander, Diksha Koul, Shubham Singh, Ravi S Manhas, Diljeet Kumar, Sanket Shukla, Ravi P N Mishra, Zabeer Ahmed, Asha Chaubey

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Article in World journal of microbiology & biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Devtulya ChanderFermentation and Microbial Biotechnology Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu, 180001, India.
Diksha KoulFermentation and Microbial Biotechnology Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu, 180001, India.
Shubham SinghBiochemical Engineering Research and Process Development Centre, CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh, 160036, India.
Ravi S ManhasBiochemical Engineering Research and Process Development Centre, CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh, 160036, India.
Diljeet KumarPharmacology Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu, 180001, India.
Sanket ShuklaPharmacology Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu, 180001, India.
Ravi P N MishraBiochemical Engineering Research and Process Development Centre, CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh, 160036, India.
Zabeer AhmedPharmacology Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu, 180001, India.
Asha ChaubeyFermentation and Microbial Biotechnology Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu, 180001, India. achaubey@iiim.res.in.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Serratiopeptidase, a proteolytic enzyme with therapeutic applications, is traditionally produced from the bacterium Serratia marcescens. Recombinant production of serratiopeptidase in Escherichia coli offers a safer alternative to the biosafety concerns of the producer. Present study involves cloning and heterologous expression of thermoactive serratiopeptidase gene from S. marcescens AD-W2 in E. coli K12 in pET28a plasmid. Optimized expression conditions i.e. 37 °C, OD

Indexed as

Peptide HydrolasesSerratia marcescensAnimalsAnti-Inflammatory AgentsBacterial ProteinsBioreactorsCloning, MolecularCytokinesEscherichia coliGene ExpressionHydrogen-Ion ConcentrationKineticsMacrophagesMiceNitric OxideRAW 264.7 CellsAnti-Inflammatory AgentsBacterial ProteinsCytokinesNitric OxidePeptide HydrolasesRecombinant ProteinsserratiopeptidaseAnti-inflammatory activityE.coli K12; serratiopeptidaseProtein refoldingTangential flow filtration

Identifiers

PMID41731164

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.